Correlation of Intraplatelet Calcium Concentration and PH with Kt/V and Blood Pressure in Maintenance Hemodialysis Patients

長期透析患者血小板內鈣離子濃度和pH與Kt/V和血壓的相關性

陳培源(Pei-Yuan Chen) ； 蔡銘川(Ming-Chuan Tsai) ； 簡孝文(Shiao-Wen Chieh) ； 陳錫賢(His-Hsien Chen)

《Acta Nephrologica》 14卷3期 (2000/07) Pp. 97-101

摘要

The aim of this study was to investigate the correlation between Kt/v, platelet intracellular calcium concentration (〔Ca²+〕i), intracellular pH (pHi) and blood pressure in maintenance hemodialysis (HD) patients. Platelet 〔Ca²+〕iand pHi were measured by fluorescent method using Fura-2 and BCECF, respectively. Platelet 〔Ca²+〕i was significantly higher (106 ± 26 vs 87 ± 18 nmole/L, p＜0.01) and pHi was significantly lower (7.11 ± 0.06 vs 7.15 ± 0.04, p＜0.01) in uremic patients than in the control subjects. Platelet 〔Ca²+〕i showed significant positive correlation (r=0.587, p＜0.02) to mean arterial pressure (MAP) and strong negative correlation (r=-0.77，p＜0.001) to Kt/V, but no significant correlation to pHi. Platelet pHi did not have any significant correlation to MAP to kt/V. however, Kt/V showed significant negative correlation (r=-0.574，p＜0.009) to MAP. Patients with Kt/V≥1.4 had significantly lowere platelet 〔Ca²+〕i (88 ± 19 vs 126 ± 17 nmol/L，p＜0.01) and lower MAP (91 ± 9 vs 110 ± 12 mmHg，p＜0.01) than did the patients with Kt/V＜1.4.
In conclusion, these results demonstrate the close interrelationship between platelet 〔Ca²+〕i, MAP and Kt/V in uremic patients on maintenance HD. Patients with higher doses of dialysis have lower platelet 〔Ca²+〕i and lower MAP before HD treatment.

關鍵字

血小板鈣離子濃度 ； 細胞pH ； 血壓 ； 透析劑量

並列摘要

The aim of this study was to investigate the correlation between Kt/v, platelet intracellular calcium concentration (〔Ca²+〕i), intracellular pH (pHi) and blood pressure in maintenance hemodialysis (HD) patients. Platelet 〔Ca²+〕iand pHi were measured by fluorescent method using Fura-2 and BCECF, respectively. Platelet 〔Ca²+〕i was significantly higher (106 ± 26 vs 87 ± 18 nmole/L, p＜0.01) and pHi was significantly lower (7.11 ± 0.06 vs 7.15 ± 0.04, p＜0.01) in uremic patients than in the control subjects. Platelet 〔Ca²+〕i showed significant positive correlation (r=0.587, p＜0.02) to mean arterial pressure (MAP) and strong negative correlation (r=-0.77，p＜0.001) to Kt/V, but no significant correlation to pHi. Platelet pHi did not have any significant correlation to MAP to kt/V. however, Kt/V showed significant negative correlation (r=-0.574，p＜0.009) to MAP. Patients with Kt/V≥1.4 had significantly lowere platelet 〔Ca²+〕i (88 ± 19 vs 126 ± 17 nmol/L，p＜0.01) and lower MAP (91 ± 9 vs 110 ± 12 mmHg，p＜0.01) than did the patients with Kt/V＜1.4.
In conclusion, these results demonstrate the close interrelationship between platelet 〔Ca²+〕i, MAP and Kt/V in uremic patients on maintenance HD. Patients with higher doses of dialysis have lower platelet 〔Ca²+〕i and lower MAP before HD treatment.

並列關鍵字

platelet calcium ； cell pH ； blood pressure ； dialysis dose