產氣膨瓶醬油之酵母菌分離、鑑定與生長抑制

Identification and Growth Inhibition of CO2-Producing Yeasts from Gas-forming Soy Sauces

黃錦城(Chin-Cheng Huang) ； 李福臨(Fwu-Ling Lee) ； 蔡維鍾(Wei-Chung Tsai) ； 王西華(Hsi-Hua Wang)

《Fungal Science》 10卷1-4期 (1995/12) Pp. 37-44

https://doi.org/10.7099/FS.199512.0037

摘要

The swelling of plastic bottles containing soy sauce was caused by CO2-producing yeasts. Four yeast species were isolated from different soy sauces and identified to be Zygosaccharomyces bailii, Zygosaccharomyces rouxii, Candida mogii, and Candida etchellsii by morphological and physiological characteristics. Among the four species, z. bailii and Z. rouxii were isolated from soy sauce containing 14% NaCl, while C. mogii and C. etchellsii were isolated from low salt (below 11% NaCl) soy sauces. In a growth inhibition study, it was found that Z. bailii and Z. rouxii could grow in the presence of 1000 ppm sodium benzoat. Z. bailii could not grow in the presence of 50 ppm butyl p-hydroxybenzoate, while Z. rouxii could not grow at a butyl p-hydroxybenzoate concentration higher than 250 ppm; C. mogii and C. etchellsii could not grow in the presence of 600 ppm sodium benzoate or 150 ppm butyl phydroxybenzoat. The four species could not grow in a mixture containing 100 ppm each of sodium benzoate and butyl p-hydroxybenzoat. In a ethanol-tolerant study, it was found that Z. bailii, Z. rouxii, C. mogii, and C. etchellsii could not grow in the presence of 2.5%, 1.5%, 2.0%, and 3.0% ethanol, respectively.

關鍵字

產氣膨瓶醬油 ； 酵母菌 ； 分離與鑑定 ； 生長抑制 ； 苯甲酸鈉 ； 對羥苯甲酸丁酯

並列摘要

The swelling of plastic bottles containing soy sauce was caused by CO2-producing yeasts. Four yeast species were isolated from different soy sauces and identified to be Zygosaccharomyces bailii, Zygosaccharomyces rouxii, Candida mogii, and Candida etchellsii by morphological and physiological characteristics. Among the four species, z. bailii and Z. rouxii were isolated from soy sauce containing 14% NaCl, while C. mogii and C. etchellsii were isolated from low salt (below 11% NaCl) soy sauces. In a growth inhibition study, it was found that Z. bailii and Z. rouxii could grow in the presence of 1000 ppm sodium benzoat. Z. bailii could not grow in the presence of 50 ppm butyl p-hydroxybenzoate, while Z. rouxii could not grow at a butyl p-hydroxybenzoate concentration higher than 250 ppm; C. mogii and C. etchellsii could not grow in the presence of 600 ppm sodium benzoate or 150 ppm butyl phydroxybenzoat. The four species could not grow in a mixture containing 100 ppm each of sodium benzoate and butyl p-hydroxybenzoat. In a ethanol-tolerant study, it was found that Z. bailii, Z. rouxii, C. mogii, and C. etchellsii could not grow in the presence of 2.5%, 1.5%, 2.0%, and 3.0% ethanol, respectively.

並列關鍵字

gas-forming soy sauce ； yeast ； isolation and identification ； growth inhibition ； sodium benzoate ； buty1 p-hydroxybenzoat