- 期刊
Isolation, Purification, and Characterization of the PR-amide Synthetase from Penicillium roqueforti
藍酪黴菌PR-amide合成酵素之分離、純化及其生化特性之探討
摘要
PR-acid舆PR-amide是藍酪黴菌所產生之次級代謝產物，由PR-amide合成酵素將PR-acid轉化成PR-amide。我們培養19天的菌絲磨出液，經ultra filtration濃縮 、80%硫酸銨鹽析，疏水性層析管柱(Phenyl)以及HPLC膠體層析管柱純化，得到最終產物活性能提高5.8倍，回收率為5.8%。經由SDS-膠體電泳分析得知此酵素分子量為66 kDa；此外，此酵素最適反應pH值為5.0，最適反應溫度為40℃。
並列摘要
PR toxin, PR-acid, and PR-amide are the secondary metabolites of Penicillium roqueforti. The chemical structures of these three compounds are closely related to each other. In an effort to understand the degradative pathway of PR toxin, we found the PR-amide synthetase. The enzyme involved in the conversion of PR-acid into PR-amide, was purified from the culture mycelium of Penicillium roqueforti ATCC48936. The enzyme has a molecular mass of approximately 66 kDa, and it is a monomer. The optimal pH for this enzyme is ca. 5.0, and the optimal temperature is 40℃.
延伸閱讀
- Chang, S. C., Cheng, M. K., & Wei, Y. H. (2004). 藍酪黴菌產生與 PR toxin 相關之代謝產物PR-imine、PR-acid 及 PR-amide 之研究. Fungal Science, 19(1&2), 39-45. https://doi.org/10.7099/FS.200406.0039
- 古庭維（2008）。納豆菌液態培養生產枯草桿菌素 NAT 最適條件之探討及其應用於乳酸球菌表現〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2008.00767
- 劉媛婷 (2010). 利用突變策略針對氧化鯊烯環化酵素進行其結構與功能及其產物專一性/多樣性之研究 [doctoral dissertation, National Chiao Tung University]. Airiti Library. https://doi.org/10.6842/NCTU.2010.00620
- 張亦雯（2013）。探討木黴菌漆氧化酵素的表達及其生化特性分析〔碩士論文，國立中正大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0033-2110201613555179
- Hsu, S. H., Hsiao, Y. Y., Liu, P. F., Lin, S. M., Luo, Y. Y., & Pan, R. L. (2009). 酵母菌表現組胺酸尾飾液泡焦磷酸水解酶之純化、定性、以及光譜分析. Botanical Studies, 50(3), 291-301. https://www.airitilibrary.com/Article/Detail?DocID=1817406X-200907-50-3-291-301-a