In the present study, sawdust wastes of ”Lentinula edodes” and ”Pleurotus sajor-caju” were used to partially replace sawdust as substrate components for the cultivation of ”Pleurotus citrinopileatus”, and were comparative study on the mycelial growth and yield. The highest mycelial growth of mushroom on five different combinative substrates was sawdust of ”Mangifera indica” (CK), followed by sawdust of M. indica mixed with sawdust waste of ”P. sajor-caju” (2:1, w/w). The crop gave a maximum biological efficiency of 38.6% on sawdust of ”M. indica” mixed with sawdust waste of L. edodes (2:1, w/w), followed by 34.1% on sawdust of M indica mixed with sawdust waste of ”P. sajor-caju” (2:1, w/w). It indicated that the substrates of sawdust of ”M. indica” supplemented with 50% sawdust waste of ”L. edodes” and of ”P. sajor-caju”, mushroom yield increased 20% and 6%, respectively, over that of sawdust of ”M. indica” (CK). From the results of this study, the substrate of sawdust of ”M. indica” supplemented with 50% sawdust waste of ”L. edodes” appeared to be the best substrate for growing ”P. citrinopileatus”.