以體外細胞染色體異常試驗評估巴西蘑菇食品安全性

Evaluation of Food Safety of Agaricus blazei Murrill Extract in vitro Chromosomal Aberration Test

吳銘芳(Ming-Fang Wu) ； 李美慧(Mei-Hui Lee) ； 常傳訓(Chuan-Hsun Chang) ； 黃幸妮(Hsing-Ni Huang) ； 敖曼冠(Man-Kuan Au) ； 劉嘉又(Jia-You Liu) ； 薛樹清(Shu-Ching Hsueh) ； 廖年捷(Nien-Chieh Liao) ； 呂旭峰(Hsu-Feng Lu)

《Journal of Biomedical & Laboratory Sciences》 24卷4期 (2012/12) Pp. 133-138

https://doi.org/10.30046/JBLS.201212.0005

摘要

對癌症病患而言，輔助與替代醫學的運用已全球化。常規醫學無法改善病情或未覺療效的病患期待，輔助與替代醫學能治癒、延長壽命、減輕症狀、強化免疫力並改善生活品質。在日本，巴西蘑菇普遍被用於治療癌症病患，但很少科學證據證明其食品安全性。為評估巴西蘑菇的食品安全性，吾輩以巴西蘑菇處理中國倉鼠卵巢細胞，研究其染色體變異情形。分別使用五種不同濃度包括5、2.5、1.25、0.625與0.3125 mg/mL處理下列中國倉鼠卵巢細胞（i）細胞加入肝臟酵素S9且與巴西蘑菇作用3小時（ii）細胞未加肝臟酵素S9且與巴西蘑菇作用3小時（iii）細胞未加肝臟酵素S9且與巴西蘑菇作用20小時。細胞經由上述不同條件處理之後，再加入秋水仙素。於細胞第一次分裂中期觀察每100個細胞具有多少個細胞具染色體異常。實驗組其染色體與染色分體構造異常包括染色體斷裂（chromosome break）、染色體缺失（chromosome deletion）、染色分體裂隙（chromatid gap）、染色分體斷裂（chromatid break）、染色分體缺失（chromatid deletion）、Y形三分染色体（triradial）、星型四分染色体（quadriradial）、環狀染色体（ring）、複雜性染色體易位（complex rearrangement）、雙中心節染色體（dicentric）、多倍染色體（polyploidy）與粉碎染色體（pulverized），共12種，少了染色體裂隙（chromosome gap）。五種濃度實驗組的不正常細胞每100個細胞約05.-3個，少於陽性對照組的12-26個。結果顯示巴西蘑菇對中國倉鼠卵巢細胞不具基因毒性。

關鍵字

輔助與替代醫學 ； 中國倉鼠卵巢細胞

並列摘要

The use of complementary and alternative medicine (CAM) has become common among many cancer patients worldwide. Cancer patients who do not improve or feel beneficial effects from ordinary medicine expect CAM to cure their disease, prolong life, alleviate symptoms, improve their quality of life and boost their immune system. Agaricus blazei is widely used among cancer patients in Japan but no scientific evidence supports their safety. In order to evaluate the food safety, we perform the chromosome aberration test in Chinese hamster ovary cells (CHO-K1) treated with Agaricus blazei Murrill extract in vitro. Different concentrations (5, 2.5, 1.25, 0.625 and 0.3125 mg/ml) of Agaricus blazei Murrill were used in the following conditions: (i) treatment by metabolic activation S9 for 3 h; (ii) treatment by no metabolic activation S9 for 3 h; and (iii) treatment by no metabolic activation S9 for 20 h. After all the above treatments, the cells were harvested after exposed to Colcemid. The frequencies of chromosomal aberrations were determined in the first metaphase after treatment. A total at 100 metaphases per treatment were scored. For the chromosome and chromatid structures alterations, the chromosome break, chromosome deletion, chromatid gap, chromatid break, chromatid deletion, triradial, quadriradial, ring, complex chromosomal rearrangement, dicentric, polyploidy and pulverized cell were all observed in the experiments displayed except chromosome gap. The numbers of abnormal cells per 100 cells in 5 experiment groups were around 0.5 to 3 cells fewer than 12 to 26 cells of positive controls. Agaricus blazei Murrill extract was not genotoxic in CHO cells.

並列關鍵字

Complem entary and altemative medicine ； Chinese ham ster ovary cells