- 期刊
高尾山蟲草無性世代（Paecilomyces tenuipes）發酵產物在抗氧化及促進吞噬能力之初步研究
A Preliminary Study on the Anti-oxidant and Phagocytosis from the Fermentation of Anamorph Paecilomyces tenuipe
摘要
蟲草屬真菌（Cordyceps spp.）是一群藥用真菌，具有廣泛的生物及藥理活性，在許多藥理學研究中可發現，蟲草屬真菌具有抗腫瘤、免疫調節、抗氧化及保肝等作用。本篇研究題材為高尾山蟲草無性世代（Paecilomyces tenuipes），探討其發酵產物在抗氧化及提升老鼠巨噬細胞（RAW 264.7）吞噬能力。實驗結果發現，P. tenuipes發酵後產物具提升巨噬細胞吞噬能力，以菌絲體熱水萃取物（1mg/mL）為最佳。熱水萃取菌絲體具有刺激老鼠巨噬細胞產生一氧化氮（NO）能力，且具有劑量遞增性效果。在抗氧化能力方面，發酵液具有不錯清除DPPH能力。再進一步使用流式細胞儀來偵測CHO-K1細胞氧化程度。結果顯示，高尾山蟲草發酵產物可降低H2O2對CHO-K1細胞內產生的氧化傷害。希望這些發現可對高尾山蟲草研究有所助益。
並列摘要
The genus Cordyceps is a group of precious medicinal mushrooms which possess a wide of biological and pharmacological activities. The medicinal effects, such as anti-tumor, immunomodulation, antioxidant activity and hepatoprotective effect, had been studied in past years. This study on the anti-oxidant and Phagocytosis from the fermentation of anamorph Paecilomyces tenuipe. The hot crude extract of mycelia could significantly enhance phagocytosis activity on RAW 264.7 (p＜0.05). Meanwhile, enhancing nitric oxide activities on macrophage cellsin adose-dependent evaluated by Griess method. In the anti-oxidized aspect, the fermentation filtrate had also shown a great activity by scavenging the DPPH. The antioxidant activity was assayed by Flow cytometry, and the result showed that the fermentation filtrate had a great antioxidant activity. These results suggest that Paecilomyces tenuipe indeed possessed some biology activities. Hopefully, these findings are useful to further studies on bioactivity of Paecilomyces tenuipe.
延伸閱讀
- 謝昆峰（2019）。以秀麗隱桿線蟲探討 Tangeretin 及 Didemethyl-tangeretin 經由 DAF-16 降低氧化壓力與類澱粉蛋白造成之毒性〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU201904172
- Huynh, T. G., & Chen, J. C. (2015). 接受馬尾藻萃取物的白蝦提升其超氧岐化酶活性與榖胱甘肽過氧化物酶活性以及對於溶藻弧菌的吞噬活力與清除效能. 臺灣水產學會刊, 42(4), 255-264. https://doi.org/10.29822/JFST.201512_42(4).0006
- 吳淑玲（2003）。褐藻酸之添加對負載萬古黴素之PLGA/幾丁聚醣微小球特性之研究〔碩士論文，中原大學〕。華藝線上圖書館。https://doi.org/10.6840/cycu200300745
- 陳桂玉、陳瑞青、张碩欽、黄建南（1996）。Isolation of Saprophytic Fungi from the Pigsty and the Effect of Disinfectant。Fungal Science，11(1&2)，49-59。https://doi.org/10.7099/FS.199606.0049
- Yang, Y. T. (2008). Production of Fructosyltransferase by Submerged Fermentation of Aspergillus japonicus [master's thesis, Tatung University]. Airiti Library. https://www.airitilibrary.com/Article/Detail?DocID=U0081-0607200917244723