Bacteria uptake nutrients from the environment via a variety of systems. The phosphoenolpyruvate: sugar phosphotransferase system (PTS) catalyzes the transport and phosphorylation of sugars or sugar derivatives into cytoplasm. The basic components of PTS are enzyme I (EI), HPr and enzyme II (EII) complex. The phosphoryl group is transferred between these components and finally to the corresponding sugar (carbohydrate). In addition to carbohydrate transportation, PTS regulates vast number of cellular processes. In this mini-review, we will describe the effects of PTS components on bacterial virulent factors, biofilm formation, bacterial resistance to antimicrobial agent and mouse infection model.