新上市流感病毒核酸快速檢測系統之效能評估

Evaluation of a New Rapid Nucleic Acid Test for Detection of Influenza Virus A&B

林桂瑩(Kuei-Ying Lin) ； 陳柏志(Po-Chih Chen) ； 梁景超(Chin-Chao Liang)

《Journal of Biomedical & Laboratory Sciences》 31卷3期 (2019/09) Pp. 77-82

摘要

近日上市一套流感病毒核酸快速檢測系統－Alere^(TM) i Influenza A&B，利用恆溫擴增反應技術原理，提供快速的流感病毒檢測。本次研究評估Alere^(TM) i Influenza A&B檢測流感病毒的效能。總共測試60件臨床剩餘檢體，以Real-time PCR做為參考方法來評估Alere^(TM) i的檢測效能。Alere^(TM) i對於A型流感的檢測敏感性為100%，專一性為95.1%；對於B型流感的檢測敏感性為93.3%，專一性為100%。結果顯示Alere^(TM) i具有相當好的檢測效能，且操作簡單，檢測時間僅需15分鐘，可提供臨床快速且準確的流感檢測結果。

關鍵字

流感 ； 快篩 ； 核酸 ； 恆溫擴增反應 ； Alere i

並列摘要

Recently, a rapid detection system for influenza virus nucleic acid, Alere^(TM) i Influenza A&B, was launched to provide rapid influenza virus detection using isothermal amplification reaction technique. This study evaluated the efficacy of Alere^(TM) i Influenza A&B in detecting influenza virus. A total of 60 clinical samples were tested and Real-time PCR was used as a reference method to evaluate the detection efficiency of Alere^(TM) i. Alere^(TM) i has a sensitivity of 100% for detection of influenza A and a specificity of 95.1%; the sensitivity for detection of influenza B is 93.3% and the specificity is 100%. The results show that Alere^(TM) i has a very good performance for detection influenza virus. Alere^(TM) i operation is simple and the detention time is only 15 minutes, which can provide clinical rapid and accurate influenza test results.

並列關鍵字

Influenza ； nucleic acid ； isothermal amplification ； Alere i