液態培養誘導黑殭菌（Metarhizium anisopliae）產孢研究

The Induction of Submerged Conidiation of Metarhizium anisopliae Study

曾敏南(Ming-Nan Tseng)

《高雄區農業改良場研究彙報》 14卷1期 (2002/12) Pp. 13-21

https://doi.org/10.6958/RBKDAIS.200212.0013

摘要

本試驗主要目的在於利用氯化鈣（CaCl2）及不同水勢（water potential）來誘導黑殭菌於液態培養環境中產生分生孢子。本試驗分別添加7.3%及24%之polyethylene glycol（PEG 200）於液態培養基malt extract broth（MEB）分別將培養基水勢調整為-2.1Mpa及-7.0Mpa，並分別添加20及40mM氯化鈣（CaCl2），於25°C、150 rpm的環境下培養5天，計算分生孢子之濃度。MEB未添加氯化鈣且未調整水勢者，培養5天之分生孢子濃度為2.39x10^5 conidia/ml；另外，未調整水勢但添加20mM氯化鈣之培養條件下，所產生之分生孢子量最高，約47.6x10^5 conidia/ml。本試驗結果發現，以MEB培養雖可產生分生孢子，但添加氯化鈣之後可明顯增加分生孢子之產量。此外，培養基水勢調整至-7.0Mpa者，無論添加氯化鈣與否，皆無法產生分生孢子。

關鍵字

黑殭菌 ； 氯化鈣 ； 分生孢子 ； 水勢

並列摘要

This study was to determine the induction of submerged conidiation of Metarhizium anisopliae var. anisopliae, using an malt extract broth. Polyethylene glycol (PEG) 200 was added at 7.3% and 24% (w/v) to achieve the water potential (Ψ) of the final broth at -2.1 Mpa and -7.0 Mpa, respectively. Also, calcium chloride (CaCl2) was added at 20 and 40 mM, respectively. Samples of M. anisopliae var. anisopliae incubated at 25°C on a rotary shaker at 150 rpm for 5 days. Conidia yields were at 2.39 × 10^5 conidia/ml in unmodified and non-calcium medium after 5 days. Adding calcium chloride at 20 mM to unmodified MEB increased the production of M. anisopliae conidia to 47.6 × 10^5 conidia/ml. However, no conidia were produced in -7.0 Mpa broth with or without calcium chloride.

並列關鍵字

Metarhizium anisopliae ； Calcium chloride ； Conidia ； Water potential