迷迭香微體繁殖與細胞懸浮培養之研究

Micropropagation and Cell Suspension Culture of Rosmarinus Officinalis

黃柄龍(Ping-Lung Huang) ； 蔡奇助(Chi-Chu Tsai)

《高雄區農業改良場研究彙報》 15卷2期 (2004/11) Pp. 33-43

https://doi.org/10.6958/RBKDAIS.200411.0033

摘要

以迷迭香葉片為培植體，培養在含有VW鹽類基本培養基，2, 4-D 5mg/l及BA 0.5mg/l中，經兩個月照光培養，可形成黃色至土黃色質地鬆軟的癒合組織；癒合組織增殖時則以含BA 2mg/l及NAA 0.2mg/l之MS培養基的效果最好。加入吸收褐色物質的吸收劑（PVP）對芽體再生有益，並可提高正常植株的形成率。細胞懸浮培養則以經0.71㎜篩網過濾的癒合組織有生長勢快速的最佳反應，可作為未來發展香精產業之應用。

關鍵字

迷迭香 ； 組織培養 ； 細胞懸浮培養

並列摘要

Leaf explants obtained from potted seedlings were used to establish an in vitro shoot organogenesis propagation system of Rosmarinus officinalis. Modified VW medium supplemented with 5mg/l 2, 4-D and 0.5mg/l BA resulted in a better textual callus after cultured in light for 2 months. When callus proliferated, it could have the best effect after cultured in MS medium containing 2mg/l BA and 0.2mg/l NAA. Addition of absorbents (PVP) were beneficial for shoots regeneration, and increasing the rate of normal plantlets regeneration. In cell suspension culture experiment, callus filtered through 0.71 ㎜ filter had the best growth respones. It could be expected to be used for the methods of future flavor industry.

並列關鍵字

Rosmarinus officinalis ； Tissue culture ； Cell suspension culture