天然雜交種蝴蝶蘭Phalaenopsis x intermedia Lindl.之起源的分子證據

Molecular Evidence for the Natural Hybrid Origin of Phalaenopsis × Intermedia Lindl.

蔡奇助(Chi-Chu Tsai) ； 黃柄龍(Ping-Long Huang) ； 陳富永(Fu-Yung Chen) ； 蘇育彥(Yu-Yen Su)

《高雄區農業改良場研究彙報》 16卷3期 (2005/09) Pp. 15-19+21-28+31-36

https://doi.org/10.6958/RBKDAIS.200509.0015

摘要

為探討Phalaenopsis × intermedia之起源，本研究分析屬於雙親遺傳的核糖體核酸（ribosomal DNA，rDNA）內轉錄間隔區（internal transcribed spacer，ITS），以及母系遺傳的葉綠體DNA的trnL基因之內隱子（intron），trnL-trnF之基因間隔區（intergenic spacer，IGS）與atpB-rbcL之基因間隔區。由ITS序列得知，P. × intermedia之單株個體所含之ITS序列具異質性（heterogeneity），主要可以分成兩大類，經群叢分析及比對樣本兩兩間之遺傳距離發現，雜交種ITS序列的兩大類分別與P. aphrodite及P. equestris之ITS序列相近，因此推斷P. aphrodite及P. equestris為P. × intermedia之親本。另外，由葉綠體DNA得知P. × intermedia與P. aphrodite最相相近，基於葉綠體DNA為母系遺傳之故，因此推斷P. aphrodite為P. × intermedia之母本，所以P. equestris則為父本。

關鍵字

天然雜交種 ； 分子證據

並列摘要

In order to confirm the hybrid origin of Phalaenopsis × intermedia Lindl., the internal transcribed spacers (ITSs) of ribosomal DNAs (rDNAs) and three fragments of chloroplast DNAs (cpDNAs) were studied. Nineteen clones of ITS sequences from three individuals of P. × intermedia Lindl. were aligned with 9 species of sections Phalaenopsis and Stauroglottis that are candidate parents. The phylogenetic tree derived from ITS data was constructed by the Neighbor-joining (NJ) method. Two major groups were shown for 19 clones of ITS sequences of P. × intermedia based on phylogenetic tree. The average genetic distance between aforementioned two groups and candidate parents was calculated based on Kimura 2-parameter method. One group has the lowest genetic distance referring to ITS repeat sequences of P. aphrodite Rchb.f., and P. equestris (Schauer) Rchb.f. is that of another. The results indicated that both P. aphrodite Rchb.f. and P. equestris (Schauer) Rchb.f. are parents of P. × intermedia based on ITS data. In addition, analysis of three fragments of cpDNAs, namely trnL intron, trnL-trnF intergenic spacer (IGS), and atpB-rbcL IGS, showed that P. × intermedia Lindl. has a lowest genetic distance with P. aphrodite Rchb.f. From both ITSs and cpDNAs data, as well as referring the property of maternal inheritance of cpDNAs, it suggested that P. aphrodite Rchb.f. is the maternal parent and P. equestris (Schauer) Rchb.f. is the paternal parent of P. × intermedia Lindl. Therefore, from these molecular evidence we support P. × intermedia Lindl. is a natural hybrid derived from P. aphrodite Rchb.f. and P. equestris (Schauer) Rchb.f.

並列關鍵字

Phalaenopsis × intermedia ； natural hybrid ； molecular evidence