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芋Colocasis esculenta癒合組織誘導與植株再生之研究
Studies on the Callus Induction and Shoot Regeneration of Taro (Colocasis esculenta)
摘要
取芋高雄一號健康種苗側芽及莖頂分生組織，培養在含有2mg/l NAA及3%蔗糖的1/2MS鹽類基本培養基，經1個月照光培養，芽體可發育形成植株。將分生苗之根、葉、葉柄及球莖部分組織切下進行癒合組織的誘導，結果僅葉柄、球莖培植體可以形成癒合組織，其中又以含有1mg/l 2,4-D及0.1mg/l BA之培養基的誘導率最高，分別為30%及55%。芽體分化時，大部分的NAA與BA或kinetin組合，極易形成根原體，只有在NAA 1 mg/l配合kinetin 0, 0.5, 2.5mg/l，其芽體誘導率分別為6.7%、16.7%、3.3%，可由癒合組織的表面形成1個至多數個數目不等的芽體，約3個月後形成一完整的植株。
並列摘要
Axillary buds and shoots tip meristem obtained from healthy plantlets that cultured in half-strength modified MS medium supplemented with 2mg/l NAA and 3% sucrose were used to establish an in vitro development for the future materials of Colocasia esculenta var. esculenta. Among different organ explants, only petiole and corm cultured in MS medium containing 1 mg/l 2,4-D, 0.1 mg/l BA and 200 mg/l glutamine for 2 months resulted in a maximum rate of callus formation. There are 30% and 55% respectively. For adventitious shoots regeneration, the most combination of NAA and BA or kinetin led to form root primordium easily. MS medium supplemented with 1 mg/l NAA and kinetin (0, 0.5, 2.5 mg/l) could induce shoot formation (6.7%, 16.7%, 3.3%). It produce lots of shoots per culture directly from the surface of calli, and the shoots could develop into intact plants with vigorous appearance after 3 months in culture.