摘要
本文主要探討不同pH 值在Aspergillus niger 生產葡萄糖氧化酵素的影響。結果發現在pH 6.0 的臨酵中胞內及胞外的葡萄糖氧化酵素活性都較高,而其最高胞內葡萄糖氧化酵素活性是pH 4.5 的4 倍。而對菌體生產的葡萄糖氧化酵素的比活性( specific activity )仍以pH6.0 較高, 其最高值為603U/g, 而pH4.5 者為60.6 U / g 。對菌體生長方面的影響,意外發現pH 4.5 的釀酵中菌體生長比較好, 在酸酵穩定期( station-aηphase) pH 4.5 的乾菌重約為pH 6.0 的1.5 倍。
並列摘要
This paper was aimed to investigate the effect of glucose oxidase production by As-pergillus niger at di 旺erent pH. The intracellular and extracellular glucose oxidase production was found higher in the fermentation at pH 6.0 than that at pH 4.5, and the maximal glucose oxidase activity at pH 6.0 was 4 times higher than at pH 4.5. The specific activity of glucose oxidase produced at pH 6.0 was higher, with the maximum at 603 U/g that is higher than 60.6 U/g at pH 4.5. However, the growth of the colony at pH 4.5 was unexpectedly found better, with the dry cell weight in the stationary phase of 1.5 times higher than that at pH 6.0.
延伸閱讀
- 林淑惠、林芊葳、莊青霖、莊偉聖（2016）。pH值對聚醯胺纖維染色影響之研究。華岡紡織期刊，23(7)，449-453。https://www.airitilibrary.com/Article/Detail?DocID=10259678-201610-201612280018-201612280018-449-453
- Shie, W. Y. (2013). 微環境酸化對於腫瘤細胞幹性維持之影響 [master's thesis, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2013.10366
- Yu, W. S. (2010). Phe427在Arthrobacter globiformis組織胺氧化酵素之受質特異性中的角色 [master's thesis, National Chiao Tung University]. Airiti Library. https://doi.org/10.6842/NCTU.2010.00074
- 李志韋、吳淑姿、余世宗（2020）。以葡萄糖與戊酸鈉為碳源於限磷條件下連續發酵培養Ralstonia eutropha生合成PHBV之探討。科學與工程技術期刊，16(1)，1-9。https://www.airitilibrary.com/Article/Detail?DocID=18166563-202003-202004010020-202004010020-1-9
- Wang, T. W. (2004). 奈米樹枝狀高分子對於細胞胞飲體及溶小體pH值的影響 [master's thesis, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2004.00685