- 期刊
Trace Analysis of Amikacin by Derivatization and High Performance Liquid Chromatography
並列摘要
A simple and sensitive liquid chromatographic method was developed for the determination of amikacin. The method is based on the detection of amikacin derivatized at amino groups with 1-naphthyl isothiocyanate. This reaction was carried out in pyridine at 70°C for 1 h. After derivatization, methylamine acetonitrile solution was added to the reaction mixture to eliminate the excess detribalizing agent. The derivative was analysed by reverse-phase HPLC on a LiChroCART Rp C18 column with water-acetonitrile (57:43, v/v) as the mobile phase, and detected at 230 nm. Several parameters affecting the derivatization of amikacin, including the reaction solvent, reaction temperature, reaction time and the amount of derivatizing agent, were investigated. The linear range of the method for the determination of amikacin was over 20-500 nmol/mL; the detection lime it (signal to noise ratio = 3; injection volume, 10 μl) was 5 nmol/mL. Application of the method to the analysis of amikacin in commercial injections has proven to be satisfactory.
延伸閱讀
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