- 期刊
Manual Solid Phase Synthesis of Potent Peptide Analogs
並列摘要
Expression of proteins in E. coins often employed to obtain protein samples; nevertheless, not all protein fragments can be cloned. Automatic and semiautomatic peptide synthesizers have been developed to efficiently synthesize peptides on a solid phase. However, the high cost limits general application for many peptide-related research projects. Therefore, we designed an experimental annd instrumental setup for the manual synthesis of various peptides and peptidomimetics. We now report the manual synthesis of a phosphopeptide SHC(pY), DDPSpYVNVQ, which is a potent liigand for the GrbZ 8HZ domain and plays an important role in signal transduction. We demonstrate that this methodology can be applied to manually synthesize various peptide analogs in a small research laboratory.
延伸閱讀
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