- 期刊
Simultaneous Determination of Ketamine, Norketamine and 6-Monoacetlymorphine in Urine Specimens by Liquid Chromatography-Tandem Mass Spectrometry: Application to a Stability Test of Various Materials
並列摘要
Aliquid chromatography-tandem spectrometry (LC-MS/MS) method to determine ketamine, norketamine and 6-monoacetylmorphine (6-MAM) in urine was developed. Ketamine-d4 was used as the internal standard. The study was performed by using a C18 column. The mobile phase consisted of 20mMammonium formate in H2O (pH 4.3, adjusted with formic acid) and ACN containing 0.1% formic acid. The gradient started at 10% organic phase and changed linearly to 85% organic phase in 2 min, after being maintained at 85% organic phase for 2 min, it then returned to the initial condition in 5 min. Ionization was conducted in the positive mode and quantification was performed by multiple reaction monitoring (MRM) mode. The within- and between-run precisions of the three analytes were less than 14.3% and that of accuracies were -7% to 12%. The retention time was 3.34, 3.28, 2.86 and 3.36 min for ketmaine, norketmaine, 6-MAM and ketamined4, respectively. The analyticalmethod was applied to a stability study of the effect of different materials of containers, polypropylene (PP), polyethylene (PE) and polystyrene (PS), on ketmaine, norketmaine, and 6-MAM at ambient temperature. The result indicated that only 6-MAM would decrease to 50% of its original concentration within 30 days in the three containers.
延伸閱讀
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