Expression of Heat Shock Cognate 70 in Breast Cancer

熱誘導同質因子70在乳癌的表現

高瑞和(Ruey-Ho Kao)

《慈濟醫學雜誌》 15卷1期 (2003/02) Pp. 21-25

https://doi.org/10.6440/TZUCMJ.200302.0021

摘要

目的:本研究之目的是要探討熱誘導同質因子70(HSC70)在乳癌之ㄧ般表現。材料與方法:我們選擇5個乳癌細胞株加上一個非乳癌乳房上皮細胞株以及7個乳癌組織加上一個正常的乳房組織來做實驗。從這些標本萃取核糖核酸，再利用一段HSC70之Cdna做為探針來進行Northetn blot 之分析。共做兩片，一片為細胞株，一片為組織標本。所得之訊號強度經過與對照強度修正後在比較。結果:在細胞株之部份，HSC70之表現在乳癌及非乳癌乳房上皮細胞株之間並無明顯差異，但其中有一乳癌細胞株T47D的表現為非乳癌乳房上皮細胞株表現的3.3倍。在組織標本方面，HSC70之表現各標本之間差異亦不大，但有一乳癌組織標本的表現是正常乳房組織表現之2.5倍。結論:大部分的乳癌和正常乳房上皮細胞具有相同程度的HSC70表現。但確實有一部份的乳癌細胞表現較高程度的HSC70。(慈濟醫學 2003; 15:21-25)

關鍵字

熱誘導同質因子70 ； 乳癌 ； 基因表現

並列摘要

Objectives; The aim of the present study was to obtain a general profile of heat shock cognate 70 gene (HSC70)expression in breast cancer. Materials and Methods: Five breast cancer cell lines plus one non-malignant breast epithelial cell line, and seven bereast cancer tissues plus one normal breast tissue sample were selected for this study. RNA was extracted from these samples using standard procedures. The HSC70 expression was assessed by Northern blot analysis using a cDNA fragment of HSC70 as the probe. Two blots, one for cell line samples, the other for tissue samples, were performed. The signal density of each sample was calibrated against a respective loading control. Results: In the bolt of cell line, the expression levels of HSC70 were similar among the breast cancer cell lines and the non-malignant cell line except for T47D, which displayed a 3.3-fold of the signal density than that of non-malignant cell line. In the blot of tissue, the variation of the HSC70 expression among the samples was narrow as well, however, one of the samples displayed a 2.5-fold greater amount of the signal density than that of the normal breast tissue counterpart. Conclusion: Most of the breast cancers and normal counterparts expressed similar levels of HSC70. However, a small fraction of the breast cancers over-expressed HSC70.(Tzu Chi Med J 2003; 15:21-25)

並列關鍵字

heat shock cognate 70 ； breast cancer ； gene expression