High Expression of the Heat Shock Cognate 70 Gene in a Cervical Cancer Cell Line

熱誘導同質因子70基因在一子宮頸癌細胞株的高度表現

高瑞和(Ruey-Ho Kao)

《慈濟醫學雜誌》 15卷4期 (2003/08) Pp. 223-227

https://doi.org/10.6440/TZUCMJ.200308.0223

摘要

目的：本研究之目的是要探討熱誘導同質因子70基因(HSC70)在一些常見癌症之表現。材料與方法：我們選擇10個分別來自於5種不同組織之癌細胞株加上3個良性細胞株來做實驗。從這些標本萃取核糖核酸，再利用一段HSC70之cDNA做為探針來進行Northern blot之分析。所得之訊號強度經過與18S核糖酸小體對照強度修正後再比較。結果：實驗結果顯示MCF7乳癌細胞株的HSC70表現度最低，而大部份標本的表現度是在MCF7乳癌細胞株的1至3倍內。在纖維芽細胞及乳腺組織，癌及非癌細胞株之間之HSC70表現度並無明顯差異。其中有一子宮頸癌細胞株HeLa的HSC70表現度異常高，為MCF7乳癌細胞株的5.9倍。結論。子宮頸癌，相對於其他腫廇，表現非常之HSC70。此HSC70高表現度與子宮頸癌形成之關係值得進一步探討。

關鍵字

熱誘導同質因子70 ； 子宮頸癌 ； 基因表現

並列摘要

Objectives: In this study, we investigated the expression of the heat shock cognate 70 gene (HSC70) in tumour cell lines and immortalized benign cell lines. Materials and Methods: Ten cell lines derived from breast cancer, colorectal cancer, melanoma, fibrosarcoma and cervical cancer, and three immortalized benign cell lines were used. RNA was extracted with standard procedures and Northem blots were probed with an HSC70 Cdna fragment. The density of the signal was measured nd calibrated against that of the respective 18S ribosome. Results: The level of HSC70 expressed in MCF7 breast cancer cells was the lowest among all cell lines. The expression level of HSC70 in HeLa cells was the highest and about 5.9 fold of that in MCF7. HSC70 levels expressed in other cell lines were about 1-3 fold of that in MCF7. Conlusion: A high level of HSC70 was expressed in HeLa cervical cancer cells. The biological significance of this requires further investigation.

並列關鍵字

heat shock cognate 70 ； cervical cancer ； gene expression