- 期刊
Molecular Biological and Epidemiological Studies of Human Cytomegalovirus Infection in Taiwan
並列摘要
We have conducted studies on human cytomegalovirus (HCMV) using a coordinated epidemiological and molecular biological approach. HCMV appears to be highly endemic in Taiwan, since 90% of residents acquire CMV infection. Many risk factors have been analyzed so far. The dominant sources of primary HCMV transmission are infected breast milk, infected genital tract, and vaginal delivery of babies. Our studies also demonstrate an association between HCMV infection and the pathogenesis of other diseases, such as hepatitis and cervical carcinoma. Our molecular biological studies aim to elucidate the transaction mechanisms of the products of two major HCMV immediate-early (IE) genes (IE1 and IE2), which regulate the expression of HCMV, and of some viral and cellular genes. pSV2Neo-derived plasmids containing the IE1 and IE2 protein, or both proteins, which are biologically active in transactivation of target gene promoters. Our immunoprecipitation study shows the interaction between IE1 or IE2 protein and some specific cellular proteins. cDNA clones encoding the 68 kDa IE1 and 82 kDa IE2 proteins were isolated, and the proteins were expressed in E. coli. the availability of large quantities of purified IE proteins will facilitate our understanding of the molecular basis of IE protein transaction, HCMV latency and reactivation.
延伸閱讀
- Wu, F. T., Wu, H. S., Yang, J. Y., Liang, S. Y., Mo, J. S., Hung, C. G., Tsao, G. C., Lin, C. Y., Lin, C. S., Su, C. Y., Shing, F. L., Hung, Y. C., & Chen, P. J. (2007). Molecular Epidemiological Investigation of Childhood Rotavirus Infection in Taiwan between 2004 and 2006. Epidemiology Bulletin, 23(8), 243-243. https://doi.org/10.6525/TEB.200708_23(8).0005
- 翁立佾（2011）。Molecular Epidemiology of Noroviruses in Northern Taiwan〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2011.02700
- 陳寶珍（2005）。Isolation and Identification of Human Metapneumovirus in Taiwan〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2005.01150
- Lee, M. H. (2010). Epidemiology and Health Risk Assessment of Hepatitis C Virus Infection in Taiwan [doctoral dissertation, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2010.00220
- Chang, Y. H. (2014). Molecular epidemiology of human parainfluenza virus type 3 in Northern Taiwan [master's thesis, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2014.03187