- 期刊
Molecular Studies of an Acrosomal Antigen Recognized by a Monoclonal Antibody and Its Application in Monitoring Sperm Acrosome Reaction
並列摘要
A conserved sperm acrosome antigen which reacts specifically with HS-11 monoclonal antibody was purified by immunoaffinity chromatography. The isolated antigen from mouse testis extract designated as MSA-11 was shown to belong to a group o fproteins with molecular mass ranging from 30 to 50 KD on SDS-PAGE. By using two-dimensional gel eletrophoresis, MSA-11 was resolved into 7 distinct proteins with isoelecric points ranging from 4.4 to 4.7. In the native state, MSA-11 was shown to be high molecular weight aggreagates as judged from Sephacryl S-300 gel filtration chromatography. Similar to HS-11 monoclonal antibody, the corresponding mouse or rabbit antisera also reacted with the acrosome of human or mouse sperm. When the monoclonal antibody was incubated with live human sperm in the capacitation medium, the percentage of methanol-fixed sperm stained with indirect immunofluorescence was found to decrease significantly with the induction of sperm acrosome reacton following the treatment with calcium ionophore A23187. The results of this study suggest that HS-11 reacts with sperm antigen which is exposed after sperm capacitaion and is shed after acrosome reaction.
延伸閱讀
- 彭文麗（2001）。Regulations of Apoptosis by Epidermal Growth Factor and Its Signal Transduction Inhibitors in Human Colorectal Cancer Cells〔碩士論文，臺北醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0007-1704200714264786
- Hsieh, K. Y. (2019). Studies on small-molecular inhibitors of tumor-associated procoagulant activity and their mechanism of action [doctoral dissertation, Kaohsiung Medical University]. Airiti Library. https://www.airitilibrary.com/Article/Detail?DocID=U0011-1808201917592000
- 林俊昌（2003）。Studies on the molecular mechanisms of Meclizine-induced cell cycle arrest and apoptosis in human colon adenocarcinoma cells〔碩士論文，臺北醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0007-1704200714513290
- 朱培倫（2004）。The Impact and Mechanism of Angiotensin II Type 2 Receptor（AT2） on Aldosterone Secretion and Adrenocortical Cell Apoptosis〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2004.01759
- Desplat, V., Dupuis, F., Trimoreau, F., Dulery, C., Praloran, V., & Denizot, Y. (1998). Effects of lipoxygenase metabolites of arachidonic acid on the growth of human mononuclear marrow cells and marrow stromal cell cultures. Mediators of Inflammation, 1998(), 31-33. https://doi.org/10.1080/09629359891351