Nucleotide Sequence of  A 5.8S Ribosomal RNA Gene and Internal Transcribed Spacer of Imperata Cylindrica (L). Beauv. VAR. Major (Cogongrass)

The entire nucleotide sequence of internal transcribed spacer (ITS) region between 18S and 26S ribosomal RNA (rRNA) genes of Cogongrass [Imperata cylindrical (L.) Beauv. Var. major (Nees) Hubb] was amplified by polymerase chain reaction (PCR). The primers for PCR, IT1-5’CGTAACAAGGTTTCC3’ and ITS2-5’ AGTTTCTTCTCCTCC were designed for amplification and sequencing. Comparing the sequence of ITS in Cogongrass with that of other higher plants, the length of PCR product was 693 bp, in which there was 67 bp for 26S rRNA, 42 bp for 18S rRNA, and 584 bp for the length of ITS region. The ITS region comprises 205 bp for ITS1, 163 bp for 5.8S rRNA and 216 bp for ITS2. In addition, between ITS1 and ITS2 there were five type repeat sequences (6 to 8 bp for each).

並列關鍵字

Cogongrass ； ITS ； rRNA ； repeat sequence

被引用紀錄

SHIH, Y. J., CHANG, H. C., TSAI, M. C., WU, T. Y., WU, T. C., KAO, P., KAO, W. Y., & CHANG, I. F. (2018). Comparative leaf proteomic profiling of salt-treated natural variants of Imperata cylindrica. TAIWANIA, 63(2), 171-182. https://doi.org/10.6165/tai.2018.63.171

延伸閱讀

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國際替代計量

Nucleotide Sequence of A 5.8S Ribosomal RNA Gene and Internal Transcribed Spacer of Imperata Cylindrica (L). Beauv. VAR. Major (Cogongrass)

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