Microseeding: Gene Transfer for Modulation of Wound Healing

Elof Eriksson

《Journal of Genetics and Molecular Biology》 13卷1期 (2002/03) Pp. 66-67

https://doi.org/10.30047/JGMB.200203.0021

並列摘要

Gene transfer has many areas of potential use in tissue repair such as skin including wounded skin, muscle, tendon, and bone. Particularly in vivo techniques, but also combinations of in vivo and in vitro techniques, are of interest to plastic surgeons.
For in vivo gene transfer to skin and wound, single subepidermal injection, the gene gun technique, or microseeding can be used. Single injection and microseeding have the advantage that not only plasma DNA, but also liposomal and viral constructs can be used. The yield is quite high, particularly when, for instance, microseeding is combined with an adenoviral vector. Gene transfer to partial thickness wounds has been done with the gene gun technique resulting in a significant acceleration of healing. For full thickness wounds, microseeding will yield high expression of protein in the wound environment. Our group has been using a chamber technique in order to contain the gene transfer process, the expressed protein, and the wound itself.
Gene transfer to bone is major interest, particularly for delivery of proteins that can upregulate the repair process in bone. Most delivery techniques call for an in vitro gene transfer protocol, usually targeting harvested bone marrow cells and these cells are autotransplanted during surgical exposure of the area that will undergo repair. Using microseeding technique, it is possible to transfect the inside of the periosteum and in this fashion, deliver proteins in high local concentrations. The technique has been used successfully in experimental research targeting the periosteum in the tibial and femoral periosteum, as well as the dento-alveolar membrane with high expression.
Our group has also worked with a tetracycline repressor system for control of timing and amount of gene expression in various cells. This system is useful, both when it is desired that the expression of the delivered gene is delayed and also allowing for more precise control of the expression of a particular gene.