Brain Tumor Gene Therapy with Tumor Suppressor/GFP Fusion Genes

Michael H. Hsiao

《Journal of Genetics and Molecular Biology》 13卷1期 (2002/03) Pp. 69-69

https://doi.org/10.30047/JGMB.200203.0023

並列摘要

Tumor suppressor gene therapy may represent a new therapeutical modality in treating malignant brain tumor. Tumor suppressor gene/green fluorescent protein (TS/GFP) fusion gene therapy vectors to suppress the growth of experimental gliomas were explored. Several fusion genes such as EGFP/PTEN and dsR1/p53 were applied alone or in combination to suppress brain tumor cell growth in vitro and in vivo. The abilities of these TS/GFP fusion genes to induce apoptosis were also investigated. The results showed high infection/expression efficiencies TS/GFP in RT-2 tumor cell line after adenovirus mediated infection at 100 MOI. DsR1/P53 or EGFP/PTEN gene induced only moderate growth suppression and apoptosis in tumor cells. However, the combination of dsR1/P53 and EGFP/PTEN induced significant growth suppression and apoptosis. Interestingly, both p53 and PTEN genes induced down-regulation of angiogenesis factor VEGF. The combination gene therapy of dsR1/p53 and EGFP/PTEN further inhibited the expression of VEGF at RNA and protein levels. In addition, significant reductions of tumor growth and blood vessel formation were detected after intratumoral injection of dsR1/EGFP and EGFP/PTEN adenoviruses. These results showed the feasibility of using combination TS/GFP gene therapy to treat malignant brain tumors.