人腦出血血腫周圍神經元凋亡及部分調控基因的研究

Neural Apoptosis of around Hematoma and Some Apoptosis-gene in Intracerebral Hemorrhage Patients

吳成翰(Cheng-Han Wu) ； 丁喜豔(Xi-Yan Ding) ； 葉小包(Xiao-Bao Ye) ； 王海燕(Hai-Yan Wan) ； 黃繩躍(Sheng-Yao Huang) ； 黃愛民(Ai-Min Huang) ； 李會忠(Hui-Zhong Li) ； 吳松鷹(Song-Ying Wu) ； 余吉(Ji Yu) ； 嚴曉華(Xiao-Hua Yan)

《內科學誌》 20卷5期 (2009/10) Pp. 440-446

https://doi.org/10.6314/JIMT.2009.20(5).08

摘要

To observe human neural apoptosis and expression of apoptosis-related genes in order to elucidate regulation mechanism in the perihematomal region of Intracerebral Hemorrhage (ICH) patients. We selected 29 specimens of the perihematoma region from 29 patients who underwent surgical evacuation of an intracerebral hematoma. The control group included 6 samples that were collected from crops within 3 hours after they died from accidents. The neural apoptosis was evaluated by terminal deoxynucleotidyl transferase-mediated deoxyuridine 5'triphosphate nick end labeling (TUNEL) method and the expression of Bcl-2, Bax, p53, and caspase-3 genes were detected by immunohistochemistry method. The apoptosis rate and the expression of Bcl-2, Bax, p53, caspase-3 in the perihematomal region of ICH patients increased significantly in comparison with the control group (p＜0.01). The expression levels of Bcl-2and p53 correlated negatively with the apoptosis rate (p＜0.01), while the expression levels of Bax, caspase-3 and the Bax/Bcl-2 ratio correlated positively with the apoptosis rate in perihematomal region of ICH patients (p＜0.01). Apoptosis was involved in the delayed brain injure after ICH in human. The expression of Bax, caspase-3 and the Bax/Bcl-2 ratio changed as the apoptosis and may play an important role during the period of apoptosis.

關鍵字

人 ； 腦出血 ； 血腫 ； 細胞凋亡 ； 凋亡基因

並列摘要

To observe human neural apoptosis and expression of apoptosis-related genes in order to elucidate regulation mechanism in the perihematomal region of Intracerebral Hemorrhage (ICH) patients. We selected 29 specimens of the perihematoma region from 29 patients who underwent surgical evacuation of an intracerebral hematoma. The control group included 6 samples that were collected from crops within 3 hours after they died from accidents. The neural apoptosis was evaluated by terminal deoxynucleotidyl transferase-mediated deoxyuridine 5'triphosphate nick end labeling (TUNEL) method and the expression of Bcl-2, Bax, p53, and caspase-3 genes were detected by immunohistochemistry method. The apoptosis rate and the expression of Bcl-2, Bax, p53, caspase-3 in the perihematomal region of ICH patients increased significantly in comparison with the control group (p＜0.01). The expression levels of Bcl-2and p53 correlated negatively with the apoptosis rate (p＜0.01), while the expression levels of Bax, caspase-3 and the Bax/Bcl-2 ratio correlated positively with the apoptosis rate in perihematomal region of ICH patients (p＜0.01). Apoptosis was involved in the delayed brain injure after ICH in human. The expression of Bax, caspase-3 and the Bax/Bcl-2 ratio changed as the apoptosis and may play an important role during the period of apoptosis.

並列關鍵字

Human ； Intracerebral hemorrhage ； Hematoma ； Cell apoptosis ； Apoptosis gene