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Curcumin Ameliorates Cerebral Ischemia-induced Oxidative Stress by Modulating Lipid Peroxidation, Antioxidant Activity, Essential Elements, Transition, and Toxic Metals

薑黃素通過調節脂質過氧化、抗氧化活性、必需元素、過渡金屬和有毒金屬來改善腦缺血引起的氧化壓力

摘要


Cerebral ischemic insult not only generates reactive oxygen species but increases oxidative stress and further cerebral injury. Curcumin is a natural polyphenol compound possesses antioxidant property. This study explored whether the neuroprotective mechanism of curcumin during cerebral ischemia is associated with the modulation of lipid peroxidation, the antioxidant superoxide dismutase (SOD), catalase (CAT), the essential element magnesium (Mg), zinc (Zn), selenium (Se), transition metal iron (Fe), copper (Cu), and toxic metal lead (Pb). Experimentally, a total of forty Sprague-Dawley male rats were used in the present study. Cerebral ischemia was induced by ligation of the right common carotid artery (RCCA) and the right middle cerebral artery (RMCA) for 1 hour. The prevention group was intraperitoneally injected with curcumin (100 mg/kg) once daily for consecutive 10 days before artery ligation. The right cerebral cortex was homogenized and the supernatants were harvested for biochemical analysis. Experimental results indicated that cerebral ischemic injury decreased SOD, CAT, Mg, Zn, Se, but increased MDA, Fe, Cu, and Pb. In turn, pretreating rats with curcumin before ischemic insult significantly reversed all biochemical results. Our findings suggest that curcumin attenuates cerebral ischemia-induced oxidative stress via modulating lipid peroxidation, anti-oxidant activity, essential elements, transition, and toxic metals. Crucially, the present results provide a new possibility for the therapeutic strategy of curcumin to chelate other neurotoxic metals.

並列摘要


腦缺血損傷會產生活性氧並導致進一步的氧化壓力與腦損傷。薑黃素是一種天然多酚化合物,具有抗氧化特性。本研究探討薑黃素在腦缺血期間的神經保護機制是否與調節脂質過氧化作用(MDA)、抗氧化物超氧化物歧化酶(SOD)、過氧化氫酶(CAT)、必需元素鎂(Mg)、鋅(Zn)、硒(Se)、過渡金屬鐵(Fe)、銅(Cu)和有毒金屬鉛(Pb)有關。本研究總共使用了40隻雄性的Sprague-Dawley大白鼠。腦缺血係透過結紮右側頸總動脈和右側中腦動脈1小時來達成。預防組則於動脈結紮前先行每日1次腹腔注射薑黃素(100 mg/kg)且連續10天。隨後再將大腦皮質均質化後,收集上清液進行生化分析。實驗結果顯示:腦缺血損傷導致SOD、CAT、Mg、Zn和Se濃度降低但MDA、Fe、Cu與Pb濃度卻升高。相反的,在腦缺血損傷之前用薑黃素預先處理則顯著逆轉了所有生化結果。我們的研究結果顯示:薑黃素通過調節脂質過氧化、抗氧化活性、必需元素、過渡金屬和有毒金屬來改善腦缺血引起的氧化壓力。重要的是,本研究結果為薑黃素螯合其它神經毒性金屬的治療策略提供了新的可能性。

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