A calibration system for helium leak calibrator is developed, and the measurement uncertainty associates with the system. The design of the system take into consideration of influencing factors that include reference standard leak calibrator (ref.), leak calibrator under testing (dut.), vacuum chamber, helium leak detector, I/O interface, and computer control. The system operates referring the standard test method for calibration of helium leak detectors by use of secondary standards (ASTM F78). The present estimate of the total uncertainty in the measured leak rate of this artifact at 23ºC at the time of test is 7.7%. This includes a systematic uncertainty of 2.0% in flow meter of the NIST primary Leak standard and 3.2% random errors in the measured leak rate of this artifact. The present research has demonstrated the high stability and great efficacy of the calibration system for helium leak calibrator.