五味子中Schizandrin及Gomisin A定量及抗肝損傷之研究

Quantitative Analysis of Schizandrin and Gomisin a and Liver Protective Activity of Schisandrae Frutctus

江睿玲(Ruey-Ling Jiang) ； 謝明村(Ming-Tsuen Hsieh) ； 周泰廷(Tai-Ting Chou) ； 郭槐秋(Huai-Chiu Kuo) ； 張永勳(Yuan-Shiun Chang)

《中醫藥雜誌》 8卷3期 (1997/09) Pp. 155-164

https://doi.org/10.6940/JCM.199709_8(3).03

摘要

五味子爲中醫常用藥材，其成分與藥理作用均被廣泛研究。本研究以水、50%酒精及95%酒精三種不同溶媒分別抽取五味子並以高效液相層析分析，比較得知以95%酒精抽取五味子其中schizandrin及gomisin A含量最高，50%酒精抽取液次之，以水抽取者含量最低。由抗肝損傷藥理試驗顯示95%酒精抽取物亦具有較高的生物活性，水抽出物活性低，可提供五味子萃取及入藥時的參考。
此外，急性毒性試驗結果亦發現五味子水、50%酒精及95%酒精三種抽取物對鼷鼠之半數致死劑量(LD50)分別爲1.60g/kg，1.75g/kg及1.00g/kg，可見五味子具有一定的毒性，使用時應注意其劑量之控制。

關鍵字

五味子 ； Schizandrin ； Gomisin A ； 定量分析 ； 抗肝損傷

並列摘要

Schisandrae Fructus, the fruit of Schisandra chinensis (Turcz.) Baill is a traditional Chinese medicine. Its constituents and pharmacological activities had been extensively studied. Schisandrae Fructus was extracted by water, 50% alcohol and 95% alcohol respectively and were quantitatively analyzed by high performance liquid chromatography (HPLC). The content of schizandrin and gomisin A in 95% alcohol extracts was highest followed by 50% alcohol extract and water extract. The 95% alcohol extracts also showed higher liver protective activity.
The acute toxicity LD50 was 1.0~l.6 g/kg (oral; 95% confidence 0.67~2.43 g/kg) of Schisandrae Fructus extract.

並列關鍵字

Schsandrae Fructus ； Schizandrin ； Gomisin A ； HPLC ； Liver protection