- 期刊
九節木之活血化瘀活性分析
Analysis on the Vasoactive-Antithrombotic Effect of Psychotria Rubra
摘要
张仲景於金匱要略中首先提出「瘀血」病名，许多研究指出，现在醫學的心血管、婦科、免疫等系统之疾病皆可用「活血化瘀」方藥治療，而血小板凝集實驗是證明藥物是否具有活血化瘀研究最重要的方法。因此，本研究以九節木「Psychotria rubra (Lour.) Poir.」作離體(in vitro)實驗，由结果顯示：(l)九節木50%酒精粗提取物(PR)在血小板懸浮液中，能约抑制U46619、Thrombin、膠原蛋白、血小板活化因子或花生四烯酸所引起的凝集，其IC50分别爲226.7、343.0、198.2、298.2、70.6 μg/ml。(2)九節木氯仿提取物(PRCHCl3)在血小板懸浮液中，能夠抑制U46619、Thrombin、膠原蛋白、血小板活化因子或花生四烯酸所引起的凝集，其IC50分别爲91.3、601.7、45.0、169.8、78.8 μg/ml。(3)九節木正丁醇提取物(PRBuOH)在血小板懸浮液中，能夠抑制U46619、Thrombin、膠原蛋白、血小板活化因子或花生四烯酸所引起的凝集，其IC50分别岛83.6、227.8、80.9、88.6、38.5μg/ml。綜合上述结果，九節和在具有活血化瘀之效，而以正丁醇提取物之效僧最高，但其有效成分的分析仍在進一步探討。
並列摘要
Psychotria rubra (Lour.) Poir. (P.R.) is one of the plant Rubiaceae. Four specious of the genus Psychotria have been found in Taiwan. Recently, the antithrombotic effect of 50% ethanolic extract of P.R. was evaluated in vitro system. The preliminary result is shown that it inhibit platelet aggregation induced by U46619, thrombin, collagen (Coll), platelet-activating-factor (PAF), and arachidonic acid (AA), with the inhibitory concentration (IC50) 226.7, 343.0, 198.2, 298.2, and 70.6 μg/mL, respectively. Then, the 50% ethanolic extract of P.R. was further partitioned with n-hexane, chloroform, and n-butanol, respectively. Again, it was found that chloroform and n-butanol fractions inhibited the U46619-, thrombin-, Coil-, PAF-, and AA-induced platelet aggregation. The IC50 of the former was 91.3, 601.7, 45.0, 169.8 and 78.8 μg/mL, and the later was 83.6, 227.8, 80.9, 88.6 and 38.5 μg/mL, respectively.