This research is to investigate the influence of the autonomic nerve in different treatment, nonacupuncture and non-acupoint acupuncture (1cm next to the Neiguan near the radial side), 30 healthy adults were tested by heart rate variability (HRV) analysis. The results indicate: in different time course, these varieties, heart rate, mean value of the RR interval, standard deviation of the RR interval, pNN50, total power, the power in the VLF range, the peak of the LF range, the power in the HF range and the maximum power in the HF range, existed significant variation. Otherwise, in the different treatment and the interaction between the treatment and the time factor, each variety has no significant variation. The result reminds us that the influence of the autonomic activity to the non-acupoint acupuncture is less obviously.