- 期刊
黃耆皂苷Ⅳ(astragaloside Ⅳ)對人類大腸癌細胞株(HT-29)細胞增殖和基質金屬蛋白酶(Matrix Metalloproteinase)基因表現的影響
The Effects of Astragaloside Ⅳ on the Cell Proliferation and Matrix Metalloproteinase Messenger RNA Expression of the Human Colon Cancer Cell Line (HT-29)
摘要
惡性腫瘤是國人十大死亡原因的首位，大腸癌躍居癌症死亡的第三位。有文獻報導大腸癌的復發或轉移和基質金屬蛋白酶MMP-2、MMP-7和MMP-9的過度表達有正相關。黃耆皂苷Ⅳ(astragaloside Ⅳ)是黃耆之主要成分。本研究結果發現黃耆皂苷Ⅳ在濃度10~100 ng/mL作用12~48小時皆可促進人類大腸癌HT-29細胞株的生長，尤其在50 ng/mL時更明顯。研究結果亦發現MMP-2 mRNA在黃耆皂苷Ⅳ濃度10及20 ng/mL時有明顯表達，但在濃度50~100ng/mL時則有抑制的作用。黃耆皂苷Ⅳ在濃度20~100 ng/mL時TIMP-1 mRNA有明顯表達，而MMP-7、MMP-9及TIMP-2皆沒有表達。
並列摘要
Malignant tumor (cancer) is the most important disease of the top ten causes of death in Taiwan. Among cancer types, colon cancer is the third most common cause of cancer-related mortality. The metastases and recurrent rate of colon cancer was correlated with the mRNA level of Matrix Metalloproteinase (MMP). Astragaloside Ⅳ is one of the important constituents of Huang-qi. In the present study, our results showed that the cell proliferation rate of HT-29 was stimulated by treatment with Astragaloside Ⅳ at 10-100 ng/mL for 12-48 hrs, especially high at 50 ng/mL. The mRNA level of MMP-2 was elevated by Astragaloside Ⅳ at 10 and 20 ng/mL. However, this level was decreased by Astragaloside Ⅳ at 50 and 100 ng/mL. The mRNA level of TIMP-1 was increased by Astragaloside Ⅳ at 20-100 ng/mL. In contrast, the mRNA levels of MMP-7, MMP-9, and TIMP-2 were not detectable in HT-29.
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