Effects of Carbon-source on the Structural Changes of Polysaccharides in the Dietary Mushroom Agaricus subrufescens

食用真菌赭麟蘑菇在不同碳源培養下菌絲多醣體之研究

趙繼嫻(Chi-Hsein Chao) ； 盧美光(Mei-Kuang Lu)

《中醫藥雜誌》 23卷1期 (2012/06) Pp. 31-40

https://doi.org/10.6940/JCM.201206_23(1).03

摘要

Agaricus subrufescens, formerly named A. blazei Murrill, has been used as dietary mushroom and to formulate nutraceuticals and functional foods. The purpose of this research was to physiochemically characterize the effects of carbon-source on the expression profiles and sugar compositions of polysaccharides from A. subrufescens. Polysaccharide was characterized by size-exclusion chromatography (SEC) and high-performance anion-exchange chromatography (HPAEC). A direct dosage effects was shown as sucrose-based medium and a negative dosage effects was shown as malt-based medium. Sucrose or glucose as substrate enhanced the biosynthesis of the very-low-molecular-weight (＜1 kDa) polysaccharide. Glucose-feeding in high dose could enhance very-high-molecular-weight-polysaccharide (＞ 10000 kDa) synthesized. In contrast, sucrose-feeding in high dose was a negative effect for that of the synthesis. Galactose, glucose, and fructose were the dominant sugars in the A. subrufescens polysaccharide mixture. The content of glucose and fructose in the polysaccharides increased with increase feeding of sucrose, and that of glucose for glucose-feeding.

關鍵字

赭麟蘑菇 ； 多醣體 ； 碳水化合物

並列摘要

Agaricus subrufescens, formerly named A. blazei Murrill, has been used as dietary mushroom and to formulate nutraceuticals and functional foods. The purpose of this research was to physiochemically characterize the effects of carbon-source on the expression profiles and sugar compositions of polysaccharides from A. subrufescens. Polysaccharide was characterized by size-exclusion chromatography (SEC) and high-performance anion-exchange chromatography (HPAEC). A direct dosage effects was shown as sucrose-based medium and a negative dosage effects was shown as malt-based medium. Sucrose or glucose as substrate enhanced the biosynthesis of the very-low-molecular-weight (＜1 kDa) polysaccharide. Glucose-feeding in high dose could enhance very-high-molecular-weight-polysaccharide (＞ 10000 kDa) synthesized. In contrast, sucrose-feeding in high dose was a negative effect for that of the synthesis. Galactose, glucose, and fructose were the dominant sugars in the A. subrufescens polysaccharide mixture. The content of glucose and fructose in the polysaccharides increased with increase feeding of sucrose, and that of glucose for glucose-feeding.

並列關鍵字

Agaricus subrufescens ； polysaccharides ； carbohydrate