The purpose of this article is to show the design and implementation process of differentiated instruction for the enhancement of Chinese reading comprehension abilities of junior high school students. Theories of differentiation were used to construct the teaching framework and instructional designs of Chinese that were applied to enrich the content. Researcher then modified learning activities according to the students' feedback and self-reflection. The process of implementation included creating the environment, designing learning content, arranging learning time and executing evaluations. The main purpose of each stage was to meet students' learning needs in order to complete the goal of enhanced Chinese reading comprehension for all students. Finally, learning effects were examined and a conclusion and recommendations were provided according to the process of design and implementation of differentiated instruction.