台灣山蘇花葉原體組織培養之研究

In-Vitro Culture of Leaf Primordium of Asplenium nidus

全中和(Jong-Ho Chyuan)

《花蓮區農業改良場研究彙報》 17期 (1999/12) Pp. 56-64

https://doi.org/10.6957/RBHDAIS.199912.0056

摘要

台灣山蘇花葉原體培養在不含生長素的MS半固體培養基上培養，三個月後可清楚看見由培植體表面長出類似癒合組織之凸起物，將這些凸起物分切數塊放入1/2MS液體培養基內懸浮培養二週，可形成兩倍的癒合組織，取這些癒合組織放入添加Benzyl adenine（BA）0.2～5 mg/l的MS半固體培養基上培養，可形成大量芽體和癒合組織。若將葉原體直接培養在添加BA 5 mg/l的MS半固體培養基上，則三個月後可清楚的看見由培植體表面形成多個芽原體，經過分切之後再放入1/2MS液體培養基內懸浮培養二週後，可形成更多類似芽原體和癒合組織，將這些組織繼代至含BA 0.2～5 mg/l的MS或1/2 MS半固體培養基上培養則會形成叢生芽體和多數癒合組織。芽體的形成以1/2MS培養基較全量MS培養基為佳，而芽體的生長以全量MS培養基較好；芽體形成的數量以添加5 mg/l BA的培養基為最多，此外，添加300 mg/l酪素水解物（casein hydrolysate）有助於芽體的生長。

關鍵字

台灣山蘇花 ； 葉原體

並列摘要

The callus-like obstrusion had been formed from the surface of the leaf primordium of Asplenium nidus in the regulator-free MS medium after three-month cultivation. The reformed obstrusion would grow as double as the old one, when it was cut into many pieces and was put into 1/2MS liquid medium with two-week suspension culture. Then these calli were put into the MS semi-solid medium supplimented with 0.2~5mg/l BA. The shoot primordium would be formed directly from the surface of the explant after three-month cultivation in the MS semi-solid medium supplimented with 5 mg/l BA. The new shoot primordia and calli would be formed and grown more and more, if the bud primordum was cut into several pieces and was cultured within the 1/2MS suspension medium about two weeks. Multiple adventitious buds and calli had been formed after cultivating these tissues in the MS or 1/2MS semi-solid medium supplimented with 0.2~5 mg/l BA. The shoots formed in the 1/2MS semi-solid medium were better than in the MS medium, however shoots grown in the MS medium were better than in the 1/2MS semi-solid medium. The amount of shoots would be greatly formed in the MS medium supplimented with 5 mg/l BA. Furthermore, it sould be much better for the shoots to grow in the medium supplimented with 250 mg/l of casein hydrolysate.

並列關鍵字

Asplenium nidus ； Leaf primordium