四季蔥健康種苗快速繁殖技術－1.分蔥潛隱病毒檢測與無病毒新梢之誘導

Rapid Multiplication of Virus-Free Vegetatively Propagated Bunching Onion (Allitum fistulosum)

楊宏瑛(Hung-Ying Yang) ； 鄧汀欽(Ting-Chin Deng) ； 張武男(Wu-Nan Chang)

《花蓮區農業改良場研究彙報》 19期 (2001/11) Pp. 39-52

https://doi.org/10.6957/RBHDAIS.200111.0039

摘要

利用免疫分析檢測分蔥潛隱病毒（SLV）在蔥苗組織內的均勻分佈，據以發展有效率的ELISA步驟，用來作青蔥健康苗繁殖體系的病毒例行檢測。試以感病之青蔥莖基盤、生長點、生長點帶一枚葉原體、生長點帶二枚葉原體等作為培植體，以培養於固態MS培養基中，只有生長點頂分生組織可獲得無病毒青蔥。對無病毒植株加速增殖方法時，TDZ與NAA同時存於MS培養基中，會減少新梢產生之數目，所以單獨添加TDZ對於誘導新梢產生之效果較佳。莖基盤經TDZ濃度1 mg/L－5 mg/L處理42日後，約可誘導43－62個新梢，即有最佳誘導新梢數目。TDZ較BA或kinetin有較佳之效果。

關鍵字

青蔥 ； 快速繁殖 ； 免疫分析 ； 分蔥潛隱病毒

並列摘要

Shallot latent virus (SLV) was detected by DTBIA, and evenly distributed in infected bunching onion (Allium fistulosum L.). An ELISA procedure was efficiently assigned for routine virus indexing in healthy seedling mass production system. For culture in MS medium, tissues from SLV infected bunching onion were excised, and the tested explants including stem-disc, apical meristem, meristem with one primordial leaf, and meristem with two primordial leaves. The results showed that only the plantlets derived from apical meristem are SLV virus-free. The rapid multiplication of healthy stock was also established. It was demonstrated that the number of regenerated shoots from stem-disc was reduced when TDZ and NAA combined together in MS media, but TDZ alone would regenerate more shoots. The stem-disc treated with 1-5 mg/L TDZ for 42 days, the optimal number of shoots (43-62) were regenerated. The TDZ had better effect on induction of shoot regeneration than BA or kinetin.

並列關鍵字

Bunching onion ； Micropropagation ； ELISA ； SLV ； Thidiazuron ； TDZ