水稻成熟種子癒傷組織誘導與再生植株之研究

Studies of Callus Induction and Shoots Regeneration from Mature Rice (Oryza sativa L.) Seeds

王啟正(Chii-Jenq Wang) ； 林學詩(Hsueh-Shih Lin)

《花蓮區農業改良場研究彙報》 21期 (2003/12) Pp. 2-13

https://doi.org/10.6957/RBHDAIS.200312.0002

摘要

為建立水稻成熟種子癒傷組織培養體系，選取22個主要水稻栽培品種進行研究，將去穎種子培養於含有2,4-Dichlorophenoxy acetic acid（2 mg/l）的N6培養基上培養，以比較水稻品種間誘導產生癒傷組織之差異。結果顯示，產生癒傷組織能力與水稻之基因型有密切的關係，稉稻品種比秈稻品種容易產生癒傷組織，其中又以台稉17號、台農67號、台農70號形成率較高，分別為96.7、96.0、97.5%。另選取台農67號、台稉4號、台稉16號、台稉17號、台中秈10號及越光等6個品種做作進一步研究。培養基添加脯氨酸可顯著增加大部分品種之癒傷組織形成率及增生量，對於癒傷組織形成率較低的品種如台中秈10號及越光等效果尤為明顯。將癒傷組織移到含有不同濃度naphthalene acetic acid（NAA）及kinetin組合的MS培養基上培養，結果顯示在培養四週後，參試之6個品種的癒傷組織均會分化出綠色芽體，惟再生能力在品種間有差異存在，台農67號、台稉4號、台稉16號及台稉17號再生能力明顯高於台中秈10號及越光。

關鍵字

水稻 ； 癒傷組織誘導 ； 植株再生

並列摘要

To establish a callus culture system from mature seeds of rice, 22 major rice cultivars in Taiwan were investigated. The dehusked seeds were incubated on the N6 medium supplemented with 2 mg/l 2,4-dichlorophenoxy acetic acid, and the differences of callus formation ability among cultivars were compared. It had shown that the callus formation ability was related to the genotype in rice. The Japonica type rice cultivars were easier to form callus than Indica type rice cultivars. Cultivars TK17, TNG67, TNG70 had higher callus formation rate than others, which were 96.7, 96.0, and 97.5%, respectively. Besides, six cultivars were chosen for further studies, which including TNG67, TK4, TK16, TK17, TCS10, and Koshihikari. For most of the investigated cultivars, medium supplemented with proline could enhance callus formation rate and proliferation amount, especially for the lower regenerating cultivars such as TCS10 and Koshihikari. After subcultured on MS medium supplemented with naphthalene acetic acid and kinetin, the calluses from six investigated cultivars were able to differentiate green shoots. However, it showed that the regeneration ability was different among rice cultivars. Cultivars TNG67, TK4, TK16, and TK17 have relatively higher regeneration ability than TCS10 and Koshikari.

並列關鍵字

Oryza sativa L. ； callus induction ； shoot regenegeration