台灣原生植物普剌特草放射線照射誘導變異之研究

Study on Gamma Ray Irradiation to Induce Lobelia nummularia Lam. Mutations

陳季呈(Chi-Cheng Chen)

《花蓮區農業改良場研究彙報》 25期 (2007/10) Pp. 53-61

https://doi.org/10.6957/RBHDAIS.200710.0053

摘要

普剌特草培植體在不含植物生長調節劑之MS培養基下生長良好，可正常發根展葉。BA具促進其芽體增殖之效果，以5 ppm BA繼代二次可獲得優質之叢生芽體。本試驗以照射劑量0、20、40、60、80 Gy處理上述之叢生芽體，結果顯示80 Gy未達半致死劑量，但培植體生長顯著受到抑制而影響其恢復，當繼代培養3個月後其發根率僅為0.9％，展葉率為7.2％，放射線照射劑量間具顯著差異。生長受到抑制之培植體外觀多呈珊瑚狀、無根，部分培植體基部呈紫紅色。本試驗結果共有15個變異枝條，變異率以40 Gy為2.3％為最高，20 Gy處理變異率為1.4％次之，且培植體生育無顯著受到抑制，故20至40 Gy之照射劑量為最適照射劑量。變異情形有葉片出現不規則之白色斑點、葉緣鑲有白邊或部分葉面呈現白色斑塊等，其中以後者變異較為穩定且最具觀賞價值，但其生長勢弱且生育緩慢。

關鍵字

普剌特草 ； 組織培養 ； 放射線 ； 誘變

並列摘要

The explants of Lobelia nummularia Lam. grew well in MS medium without any plant growth regulator applied. BA induced shoot formation of Lobelia nummularia Lam. explants. Good quality explants were obtained in BA 5ppm medium after two subcultures. Five γ-ray irradiation doses (0, 20, 40, 60, 80Gy) were used to induce mutation on Lobelia nummularia Lam. tissue culture explants. All explants survived, even under 80Gy treatment. When explants treated with 40 or higher Gy, the shoot growth and root formation were inhibited. Growth as inhibited byγ-ray irradiation treatments, resulting in coral, no root and shoot formation. There were 15 branches leaf mutations in this experiment. The highest mutation rate was 2.3% in 40Gy treatment, follow by 1.4% 20Gy. Leaves exhibited with white spots, lacings and masses in mutants. The most stable and beautiful mutants exhibited mass variegated leaves, but they were weak and grew slowly.

並列關鍵字

Lobelia nummularia Lam. ； tissue culture ； irradiation ； induced mutation