利用分子標誌輔助研發低消化性蛋白之水稻品種

Development of Low Contents of Easy-to-digest Protein Rice by Marker-assisted Selection

林泰佑(Tai-Yu Lin) ； 吳文欽(Wen-Chin Wu) ； 黃佳興(Chia-Shing Huang) ； 潘昶儒(Cheng-Zu Pan)

《花蓮區農業改良場研究彙報》 33期 (2015/12) Pp. 13-21

摘要

本研究利用DNA專一性分子標誌與SDS-PAGE單向蛋白質電泳技術進行低消化性蛋白突變Lgc1之測定並作為育種篩選之工具，期能育出具有較低易消化性穀蛋白(glutelin)含量且適應台灣氣候環境之品種。具Lgc1突變之水稻品種總蛋白質含量較一般品種無太大差異，但其蛋白質比例中榖蛋白含量與親本「春陽」相同，約為一般品種‘台稉16號’之57.5%，而不易消化之醇溶蛋白(prolamin)含量則有提升。本研究以具有低穀蛋白含量誘變基因Lgc1之日本品種 ‘春陽’與優質豐產的良質米品種‘台稉16號’雜交後代作為材料，Lgc1專一性DNA分子標誌及單向蛋白質電泳作為篩選工具，結果顯示具有同結合Lgc1訊號之品系，其蛋白質電泳分析結果顯示位於37-39 kD及22-23 kD分子量處之榖蛋白訊號呈現較弱，進行雙重分析比對後，進一步顯示該分子標誌亦適用於分析台灣稉稻品種與Lgc1品種之雜交後代，可作為台灣優良水稻品種導入低穀蛋白Lgc1突變之快速篩選工具。由具Lgc1突變品系之中針對兩親本較優異之農藝性狀進行篩選，選出具優良性狀及低穀蛋白含量之‘花稉育154號’品種，可供低腎臟負擔能力之民眾食用，深具推廣之潛力。

關鍵字

水稻 ； 低穀蛋白

並列摘要

In order to develop low gutelin content and climate adapted rice varieties in Taiwan, we analyzed low levels of easy-to digest protein mutant Lgc1 by DNA specific marker and protein contents using SDS-PAGE electrophoresis. We used the F8 generation derived from Japanese variety 'Shunyo' with Lgc1 gene and 'TK16' from Taiwan as materials for analysis. The difference in the total protein content between the Lgc1 mutant and normal rice varieties was not significant, but the proportion of glutelin contents in Lgc1 rice was the same as 'Shunyo'. The gutelin content of 'Shunyo' was reduced to 57.5% of 'TK16'. In this study, we analyzed the F8 generations by genotypes using Lgc1 specific marker and SDS-PAGE electrophoresis. The F8 lines with low gutelin contents also showed homozygosity to 'Shunyo' by genotyping. Both results of genotyping were consistant. In phenotyping, the signals of the Lgc1 lines and 'Shunyo' were weaker than which of 'TK16' in molecular weight 37-39 kD and 22-23 kD donated as glutelin. It indicated that the Lgc1 specific marker could be used as an efficient tools for detecting low gultelin content rice in marker-assisted selection. The line 'HKY154' was selected according to favorable agronomic characters and low levels of protein contents. This line has a good potential to be the food for patient with chronic kidney disease.

並列關鍵字

rice ； glutelin