In this study, Ag/SiO2/PBT master-batch was fabricated by the twin screw extruder. The concentration of Ag/SiO2 can achieve 20 wt% of the master-batch. The filter pressure test showed the master-batch was well mixed (filter pressure test, Δ P ~0 bar/kg) and the same result also showed in the SEM. The antibacterial activity against Staphylococcus aureus (S. aureus, ATCC 6358) was examined and the result showed the loading concentration>0.7 wt% of Ag/SiO2 of the fiber has an excellent performance.