製備聚丙烯腈奈米纖維離子交換膜於蛋白質純化應用

Fabrication of Electrospun Polyacrylonitrile Ion-exchange Membranes for Protein Purification

陳泰宏(T. H. Cheng) ； 周欣穎(S. Y. Chou) ； 張煜光(Y. K. Chang) ； 李宏哲(H. C. Lee)

《紡織綜合研究期刊》 23卷1期 (2013/01) Pp. 20-27

https://doi.org/10.6439/TTRJ.201301_23(1).0002

摘要

離子交換層析為一般分離純化常用的系統，但樹脂內之微孔洞易被阻塞而降低純化效果，使再生回復率下降，且樹脂本身易受壓力擠壓而破損。為決解上述問題，本研究採用薄膜分離的處理概念，結合靜電紡絲技術及快速鹼化水解程序，成功製備弱酸型離子交換奈米纖維膜。此膜材是以polyethyleneterephthalate（PET）紡黏不織布為中間基材，上下兩層為電紡polyacrylonitrile（PAN）奈米纖維膜，經由熱壓貼合成PAN-PET-PAN（AEA）膜材結構，再利用NaOH 鹼化水解PAN 奈米纖維表面之-C≡N鍵，使其變成-COOH官能基，即可製備出弱酸型離子交換奈米纖維膜（AEA-COOH）。SEM結果顯示膜材具有緻密3-D網狀且孔洞分佈集中的奈米纖維結構，奈米纖維直徑分佈均勻，平均直徑落於200-250nm之間。為了解AEA-COOH膜材特性，本研究以市售品Sartobind® C離子交換膜材為比較標準，實驗結果顯示AEA-COOH膜材的基重與膜厚分別約為Sartobind® C膜材的33%與64%，比表面積（6.1768 m^2/g）是Sartobind® C膜材的7倍，且其孔隙率（84.4%）也高於Sartobind®C膜材（73.4%），熱裂解溫度（320℃）更遠高於Sartobind® C的115℃，表示其熱穩定性高。最後，藉由進行比較不同離子交換膜材對蛋白質（Lysozyme）吸附濃度與吸附速率試驗，證實本研究利用以PAN奈米纖維所組成的弱酸型離子交換膜材會比市售品Sartobind® C膜材具有更輕、更薄、更快的吸附效率與更高的吸附濃度，以提升整體吸附分離純化的效率。

關鍵字

聚丙烯腈 ； 靜電紡絲 ； 離子交換 ； 蛋白質純化 ； 溶菌酶

並列摘要

Ion exchange (IEX) chromatography is commonly used in separation and purification systems. However, micropore blockage within its resin structure can easily lead to a reduction in the effectiveness of purification. To tackle this problem, we adopted the concept of membrane separation by combining electrospinning techniques with rapid alkaline hydrolysis to prepare a weak acid IEX nanofibrous membrane (AEA-COOH), consisting of polyethyleneterephthalate (PET) spunbond fabric as a supporting layer, with upper and lower IEX layers consisting of polyacrylonitrile (PAN) nanofibrous membranes. To determine the characteristics of the AEA-COOH membrane, we used the commercial product Sartobind® C IEX membrane as the standard of comparison. Results showed that the base weight and thickness of AEA-COOH were 33% and 64%, relative to Sartobind® C membrane. The thermo-degradable temperature of AEA-COOH membrane (320℃) was far higher than that of Sartobind® C (115℃, indicating high thermal stability. Finally, comparisons between the lysozyme adsorption rates and capacity of various IEX membranes confirmed that AEA-COOH was lighter, thinner, faster, and possessing higher protein adsorption efficiency than Sartobind®C membrane.

並列關鍵字

Polyacrylonitrile (PAN) ； electrospinning ； ion-exchange ； protein purification ； Lysozyme