近年來由於雷射應用的快速發展與成熟,促使雙光子顯微系統有突破性的成長及應用。其藉助於先進的超短脈衝雷射,而達到光學切片的能力,具有眾多的優點,包含了較少的光破壞現象以及較深的樣品穿透能力,且可以應用於活體而避免種種因傳統切片所造成的問題。上述的優點使得雙光子顯微系統在生醫光電的應用領域中具有其一定的優勢。本篇文章將針對雙光子顯微術的原理、架構以及一些本實驗室所進行生醫光電方面的應用,諸如通過皮膚藥物傳遞之機制的了解、觀測熱對膠原蛋白所產生的結構破壞以及將其應用於觀測肝硬化的現象做一簡介。
The recent rapid development of laser application has led to breakthrough like growth in two-photon microscopy. Two-photon microscopy with femtosecond pulsed laser has demonstrated the possibility of optical biopsy. The advantages of this imaging technique include less photodamage and greater imaging depth. It allows in vivo observation and avoids many problems associated with histology. The characteristics of two-photon microscopy have clear advantages in the field of biomedical imaging. This article gives an introduction to the theory and experimental setup of twophoton microscopy, and describes the application of the technology to the investigation of transdermal drug delivery, thermal damage of collagen, and liver cirrhosis.