利用原子力顯微術於液態環境下觀察DNA分子之型態變化

Imaging the Conformation Changes of Plasmid DNA Molecules in Aqueous Environment by Atomic Force Microscopy

蕭一清(I-Ching Hsiao) ； 林奇宏(Chi-Hung Lin)

《科儀新知》 149期 (2005/12) Pp. 37-43

https://doi.org/10.29662/IT.200512.0004

摘要

在過去的二十年裡，由於原子力顯微術能於液態環境下及於可控制之溫度下進行掃描，並具有能提供奈米級解析度影像之優異特性，使得有越來越多的研究學者，包含工程學家、化學家，以及生物學家，乃有興趣於其相關之應用及研究。在這篇文章中，我們將透過原子力顯微術於液態環境下觀測DNA分子貼附於經氯化鎳及APTES處理後之雲母片表面上所呈現之型態。此外，我們亦觀測到，若將DNA分子預先與氯化鎳溶液或APTES溶液混合，再滴至雲母片表面進行掃描，將導致DNA分子產生不同型態之結果。

並列摘要

For the past two decades, more and more researchers, including engineers, chemists, and biologists, were interested in the studies about the applications of atomic force microscopy in their research fields due to its capabilities that can be operated in liquid phase and at a controlled temperature, and provide nano-scale image resolution. In this study, we have observed the conformations of plasmid DNA molecules deposited on Ni- and APTES-treated mica surfaces by AFM in liquid phase. Another interesting study was also carried out in this report that DNA molecules would exhibit different conformations, while they were pre-mixed with NiCl2 solution and APTES solution before depositing on fresh cleaved mica surfaces.