- 期刊
- OpenAccess
奈米電漿子感測技術於生物分子之功能分析
Nanoplasmonic Sensing for Biomolecular Function Analysis
摘要
表面電漿子共振生物感測技術對偵測表面環境變化具有高靈敏度且不需外加任何標記等優勢，已廣泛應用於生物分子交互作用分析上。然而傳統表面電漿子共振生物感測技術仍難以直接偵測極微小分子量或極低濃度下分子間交互作用，且表面電漿子共振可提供生物分子交互作用分析之動力學資訊，但完整的生物分子辨識系統更應具有偵測構形與結構改變之能力。本研究藉由嶄新的奈米電漿子技術來解決上述三個關鍵問題。首先，利用圖樣化金奈米粒子來操控粒子電漿子或稱局域表面電漿子以提升感測器之靈敏度約十倍；再者，研發出耦合波導表面電漿子共振生物感測器，可直接即時地監測蛋白分子構形變化；最後，結合衰減全反射之表面強化拉曼散射以提供生物分子結構資訊。利用這些奈米電漿子技術，可協助快速診斷、藥物研發與蛋白質體學等研究建立一個嶄新的生物分子功能分析平台。
關鍵字
無資料並列摘要
Surface plasmon resonance (SPR) biosensing has become a standard practice in the investigation of biomolecular interaction analysis (BIA), because it is highly sensitive to the resonance condition on the sensing surface caused by environmental changes and do not require any extrinsic labeling. However, the detection limit of the conventional SPR biosensors is insufficient for the monitoring of low concentrations of small biomolecular analytes. In addition, the conventional SPR biosensor only can provide kinetic analysis information in the BIA. A more powerful biorecognition system is required not only to provide the kinetic analysis, but also to have the capability of monitoring biomolecular conformational and structural changes or trends. Therefore, in this study, nanoplasmonic technology was used to overcome three above challenges. First, patternized gold nanoparticle-enhanced plasmonic effects are utilized to manipulate particle plasmons or localized surface plasmons and enhance the biosensor sensitivity. The sensitivity of plasmonic biosensors was enhanced about 10-fold by controlling the size and volume fraction of the embedded Au nanoclusters in dielectric films. Furthermore, a coupled waveguide-surface plasmon resonance biosensor not only retains the same sensing sensitivity as that of a conventional SPR device, but also has the capability of monitoring biomolecular conformational change. Finally, with helps of attenuated total reflection surface-enhanced Raman scattering to detect the structural change of biomolecules, an advanced biomolecular recognition system with the three plasmonic techniques can provide more information in a variety of BIA. Therefore, the nanoplasmonic sensing will be a novel biosensing platform for biomolecular function analysis in fast diagnostics, drug discovery, and proteomics.