微小化細胞感測器的發展與其應用

Development and Application of Miniature Cell-Based Biosensors

蔡林彥廷(Yen-Ting Tsai Lin) ； 袁佳吟(Chia-Yin Yung) ； 陸翔寧(Hsiang-Ning Luk) ； 吳靖宙(Ching-Chou Wu)

《科儀新知》 156期 (2007/02) Pp. 68-76

https://doi.org/10.29662/IT.200702.0007

摘要

近年來結合微製程製作與微流體控制的生物感測器已廣泛地受到重視，其中又以細胞作為辨識元件之微小化生物感測器更進一步被發展成細胞測試平台，藉由微製程與微流體控制技術的整合，使微小化細胞感測器能具有快速檢測、試劑微量化與同時多功能檢測之特性。而細胞感測器的偵測原理，主要是透過量測細胞代謝率的變化或是偵測細胞膜電位的改變，以量化細胞在外源性因子刺激下的生理反應，以期能應用至評估藥物或毒物分子對人體的作用。筆者在本文中將針對細胞活性檢測原理、基材表面修飾技術與多種微型化細胞感測器的設計與實際應用做一概述。

並列摘要

Recently, the biosensor that is fabricated by microfabrication techniques with a microfluidic control has widely attracted many attentions. In particular, the biosensor that uses the cells as a recognition element is developed to form a cell-function tested platform. By using the miniaturization fabrication and the microfluidic controls, the cell-based sensors possess the properties of high throughput detection, minim reagent requirement and the multi-function measurements. The principles of the cell-based biosensor are based on the measurement of cellular metabolism rate or the detection of membrane-potential changes. The functions of the cell-based sensor can be used to quantify the physiological responses of cells under the applications of extrinsic stimulations and practically estimate the effects of drug or toxicant molecules on a human body. In the review paper, the detection principles of cellular activity, the surface modification techniques of sensor substrate, and the design and applications of various miniature cell-based sensors will be described.