以模糊滑動模態控制進行生物微流體操控與核酸萃取之研究

A Study of Bio-Microfluidic Manipulation Using Optimal Fuzzy Sliding-Mode Control

鐘永強(Yung-Chiang Chung) ； 溫博浚(Bor-Jiunn Wen) ； 葉全祐(Chuan-You Ye) ； 關壯迪(Chuang-Di Chueh)

《科儀新知》 160期 (2007/10) Pp. 91-95

https://doi.org/10.29662/IT.200710.0009

摘要

在本文中筆者敘述利用微機電(µTAS)技術來操控生物試劑的流動，並可促進生化反應的效率。文中主要是以最佳模糊滑動控制(OFSMC)為基礎，對8051作迴路設計，達到對微流道試劑的操控。其中使用的裝置包含六個部分：一部空壓機、二個流量控制器、二個流量計、一個流體驅動晶片、一個生物晶片、二個光二極體，使用晶片迴路系統來操控整個工作的情形。此種新技術在微流道內做快速核酸萃取反應，已經成功地應用在分子生物學領域。

並列摘要

In biometric and biomedical applications, a special transporting mechanism must be designed for the µTAS (micro total analysis system) to move samples and reagents through the microchannels that connect the unit procedure components in the system. In view of this, an optimal fuzzy sliding-mode control (OFSMC) based on the 8051 microprocessor was designed and a complete microfluidic manipulated biochip system was implemented in this study, with a pneumatic pumping actuator, two feedback-signal photodiodes and flowmeters for better microfluidic management. This new technique successfully improved the efficiency of molecular biology reaction by increasing the velocity of the target nucleic acid molecules, which increased the effective collision into the probe molecules as the target molecules flow back and forth.