奈米粒子於雷射脫附游離質譜分析與表面增強拉曼散射光譜之應用

Applications of Nanoparticle Matrices for Surface-Assisted Laser Desorption/Ionization Mass Spectrometry and Surface-Enhanced Raman Spectroscopy

黃銘峰(Ming-Feng Huang) ； 李昆鴻(Kun-Hong Lee) ； 林宗宏(Zong-Hong Lin) ； 江政剛(Cheng-Kang Chiang) ； 張煥宗(Huan-Tsung Chang)

《科儀新知》 165期 (2008/08) Pp. 81-89

https://doi.org/10.29662/IT.200808.0008

摘要

本文將介紹以奈米粒子作為質譜及拉曼光譜基質的分析技術。由於奈米粒子在紫外光－可見光範圍有高的吸收係數，且容易用生物分子修飾成可選擇性辨識分析物之粒子，故近年來已有許多研究專注在奈米粒子作為表面輔助雷射脫附游離化質譜儀(SALDI-MS)之基質以偵測小分子。另外，由於貴重金屬奈米粒子的電磁場增強效應明顯，故於表面增強拉曼散射光譜的原理探討與生醫偵測方面的應用也漸受重視。

並列摘要

This article briefly reviews the use of nanoparticles as matrices for the determination of small molecules using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) and as surface-enhanced Raman spectroscopy (SERS)-active substrates. It is easy to prepare bioconjugated nanoparticles that have great surface area for selective recognition of analytes of interest. In addition, nanoparticles having high molar absorptivity in the UV-Vis region are effective inorganic matrixes for the analysis of small molecules by SALDI-MS. Recently, attention has turned toward the development of SERS-active nanoparticle substrates for use as an ultrasensitive platform for the detection of biological molecules.