超大質量生物粒子的質譜研究

Mass Spectrometry of Ultrahigh-Mass Bioparticles

朱志強(Zhi-Qiang Zhu) ； 張煥正(Huan-Cheng Chang)

《科儀新知》 168期 (2009/02) Pp. 6-13

https://doi.org/10.29662/IT.200902.0001

摘要

Measuring masses of ultrahigh-mass bioparticles (m＞1MDa) has been a challenge in the field of mass spectrometry. Combining the techniques of laser-induced acoustic desorption, charge-sensitive detection, and quadrupole ion traps as the mass analyzer, we have successfully measured the masses of intact bioparticles such as viruses, bacteria, and cells in the gas phase for the first time. These particles have sizes ranging from ~100 nm to ~10 mm, with masses spanning up to 6 orders of magnitude (10^8-10^14 Da). Different from conventional mass spectrometry, we measured the masses of these bioparticles individually.

並列摘要

Measuring masses of ultrahigh-mass bioparticles (m＞1MDa) has been a challenge in the field of mass spectrometry. Combining the techniques of laser-induced acoustic desorption, charge-sensitive detection, and quadrupole ion traps as the mass analyzer, we have successfully measured the masses of intact bioparticles such as viruses, bacteria, and cells in the gas phase for the first time. These particles have sizes ranging from ~100 nm to ~10 mm, with masses spanning up to 6 orders of magnitude (10^8-10^14 Da). Different from conventional mass spectrometry, we measured the masses of these bioparticles individually.