單一粒子追蹤系統在單分子分析上的應用

The Application of Single Particle Tracking Technique in Single Molecule Analysis

張宜仁(Yi-Ren Chang) ； 邱祈翰(Chi-Han Chiou) ； 黃煜彥(Yu-Yan Huang) ； 周家復(Chia-Fu Chou)

《科儀新知》 171期 (2009/08) Pp. 32-37

https://doi.org/10.29662/IT.200908.0005

摘要

相較於傳統的群體分子量測，單分子檢測技術提供觀察分子間互動現象的機會，同時也可以從中獲得更多統計上的訊息。單一粒子追蹤技術正是單分子檢測技術中最強力的量測方式之一。本文介紹一個即時的單一粒子追蹤系統，其藉由分析微粒子的白光影像，而達到自動回授追蹤的能力。此一系統的軸向解析度小於50 nm（受限於目前硬體規格），系統反應頻率為50 Hz。藉由DNA拉扯的實驗，也證實結合磁鑷夾與單一粒子追蹤技術，可以在單分子的觀測中提供足夠的解析與應用。

並列摘要

Comparing to ensemble measurement, single molecule analysis allows us to truly observe the interaction between single molecule pair, and provides more detailed statistical information of the static and dynamic behaviors of individual molecules. Single particle tracking is one of the most powerful techniques in single molecule study. In this article, we introduce a real-time single particle tracking system which provides automatic feedback tracking of a magnetic microsphere based on its bright field diffraction patterns. The axial resolution of this tracking system is less then 50 nm, limited by our current hardwares, and its response rate is 50 Hz. We demonstrated, using DNA tethering experiment combined with magnetic tweezers, our system provides the capability of measuring molecular interactions in single molecule level.