白內障水晶體顏色係數及蛋白質濃度之測定－初報

Determination of the Color Index and Protein Concentration of the Lenses after Intracapsular Lens Extraction

鄭貞英(Jen-Eing Jeng) ； 陳振武(Chen-Wu Chen) ； 王惠珠(Hwei-Zu Wang)

《中華民國眼科醫學會會刊》 24卷1期 (1985/05) Pp. 163-173

https://doi.org/10.30048/ACTASOS.198505.0029

摘要

For determination of the color index and protein concentration of the lens gained after intracapsular lens extraction, 93 lenses of 90 cataract cases with ages ranging from 25 to 96 years, were studies on 4 soluble protein fractions: Tris-HCl buffer soluble protein, urea-soluble protein, mercaptoethanol soluble protein and NaOH soluble protein. The optic density of spectrometry and quantitative determination of protein were performed.
The Results Showed:
1. The degree of lens coloration increased with aging (P＜0.01).
2. On assay of protein concentration and the optic densimetry at 700nm, 650nm, 600nm and 500nm, it was revealed that there was a significant difference in the Tris-HCl buffer soluble protein as color of the lens nucleus increased (P＜0.05), while protein concentration and optic density of mercaptoethanol soluble protein measured at wavelengths 250nm-700nm increased with the increase of lens nucleus coloration.
3. The lens protein fraction was found to vary with age. A positive correlation was found between mercaptoethanol soluble protein fraction and patient ages (r=0.3907, P＜0.01). On the contrary, a negative correlation was noted between Tris-HCl buffer soluble protein fraction and patient ages (r=-0.4033, P＜0.01).
In conclusion, we demonstrated that the coloration of cataractous lens nucleus became deeper with aging. The distribution of protein fraction which contributed to lens coloration was found mainly in mercaptoethanol soluble protein and partly in the NaOH soluble protein.
Optic densimetry revealed that the lens substance contributing to its coloration showed prominent light absorption around at 350nm to 450nm.

並列摘要

For determination of the color index and protein concentration of the lens gained after intracapsular lens extraction, 93 lenses of 90 cataract cases with ages ranging from 25 to 96 years, were studies on 4 soluble protein fractions: Tris-HCl buffer soluble protein, urea-soluble protein, mercaptoethanol soluble protein and NaOH soluble protein. The optic density of spectrometry and quantitative determination of protein were performed.
The Results Showed:
1. The degree of lens coloration increased with aging (P＜0.01).
2. On assay of protein concentration and the optic densimetry at 700nm, 650nm, 600nm and 500nm, it was revealed that there was a significant difference in the Tris-HCl buffer soluble protein as color of the lens nucleus increased (P＜0.05), while protein concentration and optic density of mercaptoethanol soluble protein measured at wavelengths 250nm-700nm increased with the increase of lens nucleus coloration.
3. The lens protein fraction was found to vary with age. A positive correlation was found between mercaptoethanol soluble protein fraction and patient ages (r=0.3907, P＜0.01). On the contrary, a negative correlation was noted between Tris-HCl buffer soluble protein fraction and patient ages (r=-0.4033, P＜0.01).
In conclusion, we demonstrated that the coloration of cataractous lens nucleus became deeper with aging. The distribution of protein fraction which contributed to lens coloration was found mainly in mercaptoethanol soluble protein and partly in the NaOH soluble protein.
Optic densimetry revealed that the lens substance contributing to its coloration showed prominent light absorption around at 350nm to 450nm.

並列關鍵字

cataracta ； lens coloration ； lens protein