局限眼窩淋巴性腫瘤之免疫球蛋白與T細胞接受器β鏈之基因重組研究

The Study of Immunoglobulin Heavy Chain and T-Cell Receptor β Chain Gene Rearrangement in Localized Orbital Lymphoid Tumor

高淑卿(Shu-Ching Kao) ； 林秀惠(Siu-Huie Lin) ； 許紋銘(Wen-Win Hsu) ； 劉榮宏(Jorn-Hon Liu) ； 劉顯敏(Shian-Min Liu) ； 陳博明(Po-Min Chen)

《中華民國眼科醫學會會刊》 30卷2期 (1991/12) Pp. 122-130

https://doi.org/10.30048/ACTASOS.199112.0064

摘要

我們利用南方點墨雜交方法合併特定的免疫球蛋白重鏈(IgH) DNA探針與T細胞接受器β鏈(TCRβ) DNA探針來研究九例局限眼窩淋已性腫瘤的IgH與TCR β基因重組形式。在九例病理與免疫表現型檢查都屬良性多株性的眼窩淋巴腫瘤中，有七例呈現株聚性IgH基因重組(clonal IgH gene rearrangement)。這七例在平均21.4個月之追蹤期，沒有病例有眼窩或是全身性惡性淋腫瘤的發現；若以此觀點來論，基因的單林性(genetic monoclonality)並不可將其視為惡性腫瘤的一個基因標記(genetic marker)。然而良性多株性眼窩淋巴性腫瘤含有單株B細胞增殖的表現(monoclonal β cell population)，可能隱藏會轉化成惡性淋巴腫瘤的危機；因此對於有單株IgH基因重組的良性眼窩淋巴性腫瘤患者，系列的追蹤與定期的血液方面之檢查，是有其必要的。

並列摘要

We investigated the organization of the immunoglobulin heavy chain (IgH) and the T cell receptor beta chain (TCRβ) gene loci in nine orbital lymphoid masses which classified as benign, polyclonal lymphoid tumor by histopathologic and immunophenotypic examinations. Seven of these nine benign, polyclonal lymphoid tumors exhibited clonal IgH gene rearrangement. None of the seven patients in this series carrying such lesion developed overt clinical evidence of lymphoid malignancy at follow up period. From this point view, It should be underscored the genetic monoclonality does not equate with malignancy or systemic disease.
However, clonal β cell expansion within benign, polyclonal lymphoid tumor may imply the risk of monoclonal β cell lymphoid transformation. Therefore, lougitudinal studies and periodic hematologic examinations of patient with clonal IgH gene rearrangement are necessary.

並列關鍵字

Immunoglobulin heavy chain ； T cell receptor beta chain ； Clonal gene rearrangement ； Orbital lymphoid tumor ； Southern blot hybridization