Human Adult Lens Epithelial Cells with Morphological Manifestations Suggestive of Differentiation in Culture

成人水晶體上皮細胞在培養中呈現顯示分化之形態變化

葉則祥(Tse-Shiang Yeh) ； 張武修(Wushou P. Chang) ； 蔡長祐(Chang-Youh Tsai) ； 黃嵩立(Song-Lih Huang) ； 張由美(Yu-Mei Chung) ； 劉瑞玲(Catherine J. L. Liu) ； 黃呈榕(Cheng-Jung Huang) ； 李淑美(Shui-Mei Lee) ； 劉榮宏(Jorn-Hon Liu)

《中華民國眼科醫學會雜誌》 32卷4期 (1993/12) Pp. 456-461

https://doi.org/10.30048/ACTASOS.199312.0011

摘要

於體內，座落於水晶體前囊的赤道前部的上皮細胞能進行分裂和分化以形成水晶體纖維。因其解剖上的特性，牠們適合作為有關生長，分裂和分化等科學研究之用。過去文獻中曾描述在人類胎兒或動物水晶體上皮細胞培養中可觀察及研究其分化現象。我們於此描述在培養自有白內障的老人取得的水晶體上皮細胞中觀察到的分化現象。自有較輕微白內障的水晶體取得的上皮細胞可能呈現顯示分化之形態變化。可能出現拉長的柱狀細胞，有細長放射狀突出的星狀細胞或甚至線狀、纖維般的細胞。偶而，細胞能聚集形成類晶體球體。此種培養可為進一步研究成人水晶體纖維細胞之分化過程提供材料。

並列摘要

In vivo, the lens epithelia located preequatorially on the anterior capsule may undergo mitosis and differentiate into new lens fibers. Due to anatomical characteristics, they are suitable for scientific researches on growth, mitosis and differentiation. Signs of differentiation have been demonstrated and studied in cultures of explanted human fetal or animal lens epithelia in previous reports. We herein describe those in cultures of adult lens epithelial cells derived from aged patients with cataract. During the growth of the cells from lenses with milder cataract, morphological manifestations suggesting differentiation might be noted which consisted of elongated rod-shaped cells, stellate-like cells with elongated radiating processes or even thread-shaped, fiberlike cells. Occasionally the cells might aggregate to form lentoid bodies. Such cultures might provide materials for further study on the differentiation of adult lens fibers.

並列關鍵字

human lens epithelial cells ； elongation ； differentiation ； tissue culture